敬钊缨毛蛛毒素对小鼠脑缺血再灌注损伤神经保护作用  

Neuroprotective effect of Chinese tarantula Chilobrachys jingzhao venom on cerebral ischemia- reperfusion injury in mice

作　　者：米锡阳[1] 史小军[1] 王梧霖[1] 

机构地区：[1]清华大学生命科学学院,深圳518055

出　　处：《中华神经医学杂志》2011年第10期1005-1008,共4页Chinese Journal of Neuromedicine

基　　金：深圳市创新资源集聚计划国际科技合作研发项目（ZYA200903270135A）

摘　　要：目的探讨敬钊缨毛蛛毒素对小鼠脑缺血再灌注损伤的神经保护作用。方法20只小鼠按随机数字表法分为4组：正常组、假手术组、模型组、蜘蛛毒素组，每组各5只。应用大脑中动脉栓塞法制备小鼠脑缺血再灌注损伤模型。TTC染色检测脑梗死体积，比色法检测血清超氧化物歧化酶（SOD）、丙二醛（MDA）水平，RT-PCR和免疫组化染色分析环氧化酶-2（COX-2）mRNA和蛋白的表达变化。结果与模型组相比，蜘蛛毒素组脑梗死体积明显减小，血清SOD活性明显增加，MDA表达水平明显降低，COX-2mRNA和蛋白表达水平明显下降，差异均有统计学意义（P〈0．05）。结论敬钊缨毛蛛毒素对脑缺血再灌注损伤有神经保护作用，其机制可能是提高脑缺血后细胞抗氧化能力和下调COX-2表达量。Objective To investigate the effect of Jingzhaotoxin （JZTX） from Chinese tarantula Chilobrachys jingzhao venom on cerebral ischemia/reperfusion injury in mice. Methods Twenty mice were equally randomized into normal group, sham-operated group, vehicle group and JZTX treatment group （n=5）. Cerebral ischemia-reperfusion injury was induced by middle cerebral artery occlusion. Cerebral infarct volume was measured by TTC staining. The superoxide dlsmutase （SOD） activity and malonaldehyde （MDA） content in serum were detected with colorimetric method. Immunohistochemistry and real-time PCR were used to analyze the expressions of cyclooxygenase-2 （COX-2）. Results The cerebral infarct volume in the JZTX treatment group was significantly decreased as compared with that in the vehicle group （P〈0.05）; higher SOD activity and lower MDA content in the JZTX treatment group after ischemic insult were noted than those in the vehicle group （P〈0.05）; the mRNA and protein expressions of COX-2 in the JZTX treatment group was significantly down-regulated as compared with those in the vehicle group （P〈0.05）. Conclusion JZTX has neuroprotective effect in cerebral ischemia/reperfusion injury, whose mechanism might be related to the improvement of antioxidant capacity and the down-regulation of COX-2 expressions after cerebral ischemia.

关 键 词：蜘蛛毒素 脑缺血 超氧化物歧化酶 丙二醛 环氧化酶-2 

分 类 号：R743.3[医药卫生—神经病学与精神病学]