白腐真菌Pleurotus sajor-caju预处理对红麻秸秆发酵乙醇的影响  

Effects of microbial pretreatment of kenaf stalk by the white-rot fungus Pleurotus sajor-caju on bioconversion of fuel ethanol production

作　　者：阮奇城[1,2] 祁建民[1,2] 胡开辉[2] 方平平[1] 林海红[1,2] 徐建堂[1] 陶爱芬[1] 林国龙 易利福 

机构地区：[1]福建农林大学教育部作物遗传育种与综合利用重点实验室,福州350002 [2]福建农林大学能源植物研究中心,福州350002 [3]福建省曲斗香酒业有限公司,泉州362400

出　　处：《生物工程学报》2011年第10期1464-1471,共8页Chinese Journal of Biotechnology

基　　金：现代农业产业技术体系建设专项(No.nycytx-19-E05);国家农业部公益性行业(农业)科研专项(No.nyhyzx-018(3));泉州市科技重大项目(No.2008ZD12-6)资助~~

摘　　要：为研究微生物法预处理对红麻秸秆中木质素的降解及后续的红麻纤维素酶促糖化和发酵效率的影响,将白腐真菌Pleurotus sajor-caju接种在红麻秸秆培养基上固态培养,对红麻秸秆进行预处理。经P.sajor-caju培养25~35 d后,有效转化红麻秸秆中的木质素,转化率最高可达50.20%,并提高红麻纤维素的酶促水解效率,糖化率达69.33%~78.64%,与对照组相比提高了3.5~4.1倍。以微生物法预处理后的红麻秸秆样品为底物的同步糖化发酵实验表明,发酵72 h,发酵液中乙醇浓度达到18.35~18.90 mg/mL,最高乙醇产率达到理论产率的68.31%。Kenaf stalk was pretreated by the white-rot fungus Pleurotus sajor-caju incubated in solid-state kenaf stalk cultivation medium.Delignification and subsequent enzymatic saccharification and fermentation of kenaf stalk were investigated in order to evaluate effects of microbial pretreatment on bioconversion of kenaf lignocellulose to fuel ethanol production.The highest delignification rate of 50.20% was obtained after 25？35 days cultivation by P.sajor-caju,which could improve subsequent enzymatic hydrolysis efficiency of kenaf cellulose.And the saccharification rate of pretreated kenaf stalk reached 69.33 to 78.64%,4.5？5.1 times higher than the control.Simultaneous saccharification and fermentation（SSF） with microbial-pretreatment kenaf stalk as substrate was performed.The highest overall ethanol yield of 68.31% with 18.35 to 18.90 mg/mL was achieved after 72 h of SSF.

关 键 词：红麻秸秆 白腐真菌 微生物预处理 木质素降解 同步糖化发酵 纤维素乙醇 

分 类 号：TQ223.122[化学工程—有机化工]