层黏连蛋白和纤维连接蛋白在肾发育中的表达  

Expression of laminin and fibronectin in developing mouse kidney

作　　者：张萍[1] 宋小峰[1] 郭敏[1] 

机构地区：[1]辽宁医学院组织学与胚胎学教研室,锦州121001

出　　处：《解剖学杂志》2011年第5期626-629,共4页Chinese Journal of Anatomy

基　　金：辽宁省教育厅资助项目（2009A454）

摘　　要：目的：观察层黏连蛋白（LN）和纤维连接蛋白（FN）在小鼠肾发育中的时空性表达，探讨其与肾发育的关系。方法：采用免疫组织化学技术，结合免疫印迹法，检测不同胚龄及生后日龄小鼠肾组织LN和FN的表达及其含量变化。结果：LN在输尿管芽、各期肾小体、肾小管及集合管的基底膜处均有表达，并随肾的发育其表达量逐渐增加；FN在除逗号小体以外的各期肾小体、肾小管和集合管的基底膜处均有表达，随肾的发育其表达量逐渐增加。结论：LN和FN可能对小鼠肾发育以及成熟肾各结构的维持起重要作用。Objective： To observe the spatiotemporal expressions of laminin （LN） and fibronectin （FN） in developing mouse kidney and to investigate the relationship between the expression and kidney development. Methods： Immunohistochemistry and Western blot were used to observe and measure the expression of LN and FN in embryos and postnatal mouse kidneys. Results： The expression of LN was noticed in the ureteric bud, all stages of glomeruli, renal tubules and collecting ducts, and was increasing gradually with ages; The expression of FN was detected also in all stages of glomeruli, renal tubules and collecting duets, except for the commar-shaped bodies, and also increased gradually with ages. Conclusion： LN and FN play an important role in the development and maturation of the mouse kidney,

关 键 词：肾发育 层黏连蛋白 纤维连接蛋白 免疫组织化学 小鼠 

分 类 号：R575.2[医药卫生—消化系统]