小鼠骨髓源性内皮祖细胞培养鉴定及7．0T磁共振成像研究  

Identification and 7.0 T magnetic resonance imaging of murine bone marrow-derived endothelial progenitor cells

作　　者：陈蓉[1] 余辉[1] 贾振宇[1] 滕皋军[1] 

机构地区：[1]东南大学附属中大医院放射科东南大学江苏省分子影像与功能影像重点实验室,南京210009

出　　处：《中华医学杂志》2011年第37期2644-2647,共4页National Medical Journal of China

基　　金：基金项目：国家自然科学基金（30870703）

摘　　要：目的应用纳米磁粒子Resovist体外标记小鼠骨髓源内皮祖细胞（EPCs），磁共振（MR）对标记细胞成像。方法分离小鼠骨髓单个核细胞，免疫荧光鉴定细胞表面标记CD31、KDR，检测对DiI标记的乙酰低密度脂蛋白（DiI-AcLDL）内吞和FITC标记的荆豆凝集素-1（FITC—UEA-1）结合功能。氧化铁纳米颗粒（Resovist）标记EPCs，普鲁士蓝染色显示细胞内铁。原子吸收光谱仪检测溶液内铁含量。7．0TMR进行标记细胞成像。结果小鼠骨髓单个核细胞体外培养培养7d时，克隆增大，梭形细胞增多；培养14d时，细胞生长密集呈铺路石状。免疫荧光显示表达内皮细胞标志CD31、KDR，能结合UEA、内吞LDL，普鲁士蓝染色显示Resovist细胞标记率接近100％。原子吸收光谱仪检测标记后细胞内铁浓度为2．33ug／ml。MRI显示标记Resovist的细胞较未标记者E梯度回波序列信号降低。结论小鼠骨髓源内皮祖细胞Resovist标记后MR可进行细胞群成像，用于进一步细胞治疗及实时活体监测。Objective To label murine bone marrow derived endothelial progenitor cells （EPCs） with nanometer magnetic probe （Resovist） and perform magnetic resonance （MR） imaging on these labeled cells. Methods Murine bone marrow derived mononuclear cells （MNCs） were isolated. And EPCs were selected by adherence method, expanded and incubated with Resovist. Cell surface markers were detected with immunofluorescence. Endocytosis of Dil-AeLDL （dil-labeled aeetylated low density lipoprotein） and in vitro binding of FITC-UEA-1 （fluoresceinisothiocynate-vicia ervilia lectin-1 ） were also detected. Intracellular iron was detected by Prussian blue dye. Different quantities of labeled cells were visualized by 7.0T MR. Results The cultured cells expressed the endothelial cell markers of CD31 and KDR with immunofluorescence. The cells could bind UEA and endocytosize LDL. Iron particles could be observed in cells with Prussian blue dying and the ratio of label was approximately 100 percent. The Resovist labeled cells showed a lower signal than the non-labeled counterparts on T2＊ WI （weighted imaging）, especially the 1 × 10^6 labeled cells showed the lowest signal. Conclusions Murine bone marrow derived MNCs can be induced into EPCs and Resovist can label them effectively. The labeled cells can be visualized in vitro by 7.0T MR for cellular therapy and real time in vivo monitor.

关 键 词：干细胞 内皮 血管 磁共振成像 

分 类 号：R730.2[医药卫生—肿瘤]