内毒素休克大鼠血清中TNF-α、皮质醇、LPS的测定及肾上腺的病理改变  

Detection of serum TNF-α,corticosteroid,LPS and pathological changes of adrenal gland tissue in endotoxic shock rats

作　　者：孙佳[1] 夏海鸣[2,3] 

机构地区：[1]东南大学医学院传染科,江苏南京210009 [2]东南大学附属第二医院,江苏南京210003 [3]东南大学医学院内科学,江苏南京210009

出　　处：《东南大学学报（医学版）》2011年第5期728-732,共5页Journal of Southeast University(Medical Science Edition)

基　　金：南京市科技发展计划项目资助(200901093);南京市卫生局医学重点科技发展项目资助(ZKX08033)

摘　　要：目的:建立内毒素休克大鼠模型,探讨内毒素休克形成机制。方法:将40只Wistar大鼠随机分为正常对照组(n=10)和内毒素休克组(n=30)。内毒素休克组采用腹腔注射适量D-氨基半乳糖(D-GalN)致敏后,经尾静脉注射休克剂量内毒素脂多糖(LPS),观察大鼠平均动脉压(MAP)变化,以评定模型是否成功。ELISA法测定血清中肿瘤坏死因子(TNF-α)、血清及肾上腺组织中LPS浓度,电化学放光免疫分析法测定血清中皮质醇浓度,光镜下观察肾上腺组织的形态学改变。结果:注射休克剂量的LPS后,大鼠MAP迅速下降,回升至正常后逐渐缓慢下降并维持较低水平,其中19只大鼠符合休克标准,其血清中TNF-α、皮质醇、LPS的质量浓度分别为842.41 ng.L-1、9.50μg.L-1、231.44μg.L-1,肾上腺组织中的LPS质量浓度为154.23μg.L-1。光镜下可见肾上腺组织有明显充血和水肿。结论:LPS、TNF-α参与了内毒素休克的发生、发展过程。休克大鼠的血清皮质醇浓度明显升高,并伴有肾上腺组织的病理改变。Objective： To detect serum TNF-α,corticosteroid,LPS level and adrenal glands tissue LPS level by producing the rat models with endotoxic shock and to observe histological changes of adrenal gland tissue with microscopy.Methods： Forty Wistar rats were randomly divided into two groups： normal control group（n=10）,endotoxic shock group（n=30）.Endotoxic shock group were sensitized by D-GalN by intraperitoneal injection,then given LPS by tail vein injection.Changes of mean arterial pressure（MAP） were observed to evaluate the animal model.Serum TNF-α concentration,serum and adrenal glands tissue LPS concentrations were measured by enzyme-linked immunosorbent assay（ELISA）.Serum corticosteroid concentration was detected by electro-chemiluminescence immunoassay（ECLI）.Histological alterations of adrenal glands tissue were studied with microscopy.Results： The MAP decreased obviously after injection of LPS,then recovered to normal,and decreased slowly,and kept at a low level.Nineteen rats corresponded shock standards,whose serum TNF-α,corticosteroid and LPS concentrations were respectively 842.41 ng·L-1,9.50 μg·L-1,231.44 μg·L-1,and adrenal glands tissue LPS concentration was 154.23 μg·L-1.Pathological changes of adrenal glands tissue presented congestion and dropsy.Conclusion： LPS,TNF-α involves in the genesis and development of endotoxic shock.Serum corticosteroid of endotoxic shock rats increases obviously accompanied by pathological changes of the adrenal glands tissue.

关 键 词：内毒素休克 脂多糖 肿瘤坏死因子 皮质醇 肾上腺 大鼠 

分 类 号：R364.14[医药卫生—病理学] R515[医药卫生—基础医学]