GLP-2对SAP大鼠肠黏膜屏障的保护作用  

Protective effects of GLP-2 on intestinal mucosal barrier in rats with severe acute pancreatitis

作　　者：陈霞[1] 赵宏贤[2] 王忠琼[1] 李昌平[1] 

机构地区：[1]泸州医学院附属医院消化内科,四川泸州646000 [2]泸州医学院组胚教研室,四川泸州646000

出　　处：《重庆医学》2011年第31期3127-3128,3131,F0002,共4页Chongqing medicine

基　　金：四川省卫生厅科研课题(070267)

摘　　要：目的探讨胰高血糖素样肽-2(GLP-2)在重症急性胰腺炎(SAP)肠黏膜屏障中的保护作用。方法 SD大鼠30只,随机分为3组:假手术组、SAP组、GLP-2组。采用胰管逆行注射3%牛磺胆酸钠溶液制作SAP模型。GLP-2组在制作SAP模型后,皮下注射GLP-2 0.1mg/kg,连续注射3d。3d后处死大鼠。透射电子显微镜观察小肠黏膜改变,检测各组血浆二胺氧化酶(DAO)及内毒素(ET)水平。结果 SAP组大鼠小肠黏膜出现明显病理损害;与SAP组相比较,GLP-2组小肠黏膜损伤显著减轻。SAP组DAO及ET水平明显高于假手术组(P<0.05);与SAP组比较,GLP-2组DAO及ET水平明显低于SAP组(P<0.05)。结论 GLP-2对SAP大鼠肠黏膜屏障功能具有一定保护作用。Objective To explore the protective effects of glucagon like peptide-2（GLP-2） on intestinal mucosal barrier in rats with severe acute pancreatitis（SAP）.Methods 30 rats were randomly divided into 3 groups：control group（sham operation group）,severe acute pancreatitis model group（SAP group）,GLP-2 group（SAP rats treated with GLP-2）.The SAP rat models were established via retrograde injection of 3% sodium taurocholate to the pancreatic duct.Rats in GLP-2 group were peritoneally injected with GLP-2 with dose of 0.1 mg/kg per day for 3 days.Three days later,the severity of intestinal mucosal damage was investigated by transmission electron microscope（TEM）.Plasma concentration of diamine oxidas（DAO） and entotoxin（ET） in each rat were determined.Results Compared with control group,the intestinal mucosa of rats in SAP group injured significantly.GLP-2 treatment attenuated AP-induced mucosal injury.Compared with control group,the concentration of DAO and ET in SAP group increased significantly（P0.05）;Compared with SAP group,the concentration of DAO and ET in GLP-2 group decreased significantly（P0.05）.Conclusion GLP-2 has a protective effects on intestinal barrier in rats with severe acute pancreatitis.

关 键 词：胰高血糖素样肽-2 肠黏膜 胺氧化酶 内毒素 

分 类 号：R576[医药卫生—消化系统]