索拉非尼联合热疗对肝癌HepG-2细胞中Bax、Bcl-2表达的影响  

Effect on Bax and Bcl-2 Proteins Expression on Human Hepatocellular Carcinoma Cells Line HepG-2 by the Treatment of Sorafenib Combined with Hyperthermia

作　　者：肖广显[1] 丁素银[2] 程英俊[2] 李青山[3] 丁素静[2] 

机构地区：[1]解放军252医院急诊外科,河北保定071000 [2]保定恒兴中西医结合医院肿瘤内科,河北保定071000 [3]承德医学院附属医院肿瘤放化疗科,河北承德067000

出　　处：《解放军医药杂志》2011年第5期1-4,I0002,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

摘　　要：目的探讨索拉非尼联合热疗对人肝癌HepG-2细胞株中Bax及Bcl-2蛋白表达的影响。方法将实验细胞分为4组:对照组(正常培养的HepG-2细胞)、索拉非尼组(索拉非尼药物浓度为12μmol/L)、热疗组(加热温度为43℃,加热时间为2 h)及索拉非尼联合热疗组。用免疫组织化学染色法观察各组人肝癌HepG-2细胞株中Bax及Bcl-2的表达情况。结果肝癌HepG-2细胞Bax表达于胞浆和(或)胞膜,Bcl-2主要表达于胞膜,均呈棕黄色弥漫状分布;索拉非尼及热疗均使人肝癌HepG-2细胞中Bax蛋白的表达有所增加(P<0.01),且均使Bcl-2蛋白的表达有所降低(P<0.01),与索拉非尼组及热疗组化较,索拉非尼联合热疗组Bax蛋白的表达明显增加(P<0.01)、Bcl-2蛋白的表达明显降低(P<0.01)。结论索拉非尼联合热疗通过增加人肝癌HepG-2细胞中Bax蛋白的表达和降低Bcl-2蛋白的表达协同诱导肝癌细胞凋亡,可能成为治疗肝癌的新方法。Objective To observe the effect on Bax and Bcl-2 proteins expression on human hepatocellular carcinoma HepG-2 cells by the treatment of Sorafenib combined with hyperthermia.Methods The HepG-2 cells were divided into 4 groups： control group（normal cultured HepG-2 cells）,Sorafenib group（concentration of 12 μmol/L）,hyperthermia group（43℃,2 h） and Sorafenib combined with hyperthermia group.Bax and Bcl-2 proteins expression on HepG-2 cells was examined with immunohistochemistry technique.Results Bax was expressed on cytoplasm and（or） envelope,Bcl-2 was expressed on Bcl-2,and both were buffy and asystematic.The levels of Bax proteins were lower in Sorafenib group and hyperthermia group when compared with those in the control group,and the levels of Bcl-2 proteins were lower in Sorafenib group and hyperthermia group when compared with those in the control group,and the differences were significant（P0.01）.Compared with those of Sorafenib group and hyperthermia group,Bax proteins increased in Sorafenib combined with hyperthermia group,but Bcl-2 proteins were lower（P0.01）.Conclusion Sorafenib combined with hyperthermia may increase the expression of Bax and the decrease the expression of Bcl-2 proteins and induce HepG-2 cells into apoptosis.It is a new approach in the treatment of primary liver cancer.

关 键 词：肝肿瘤 HEPG-2细胞 索拉非尼 透热疗法 基因 BCL-2 基因 Bax 基因表达调控 肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]