HPLC测定天麻壮骨丸中天麻素的含量  

Determination of Gastrodin in Tianma Zhuanggu Pills by HPLC

作　　者：陈志伟[1] 米之金[1] 张康[1] 文永盛 

机构地区：[1]西华大学生物工程学院,成都610039 [2]成都市食品药品检验所,成都610044

出　　处：《中国实验方剂学杂志》2011年第21期116-118,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立天麻壮骨丸中天麻素的高效液相含量测定方法。方法:色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm),以乙腈、磷酸水溶液为流动相进行梯度洗脱,检测波长220 nm,流速1 mL.min-1,柱温25℃。结果:天麻素在0.118 8～2.376μg呈良好的线性关系,平均加样回收率为99.6%,RSD 2.44%。结论:方法快速、简便、准确、重复性好,结果可靠,可为天麻壮骨丸的质量评价提供有效手段。Objective：To estalish an accurate methed for the determination of Gastrodin in Tianma Zhuanggu Pills.Methed： Diamonsil C18（4.6 mm×250 mm,5 μm）column in an oven at 25 ℃ was need,with a gradient elution which consist of acetonitrile and phosphoric acid solution and a UV detector at 220 nm,the flow rate was 1.0 mL·min-1.Result： The linear ranges of Gastrodin was 0.118 8-2.376 μg.The average recovery was 99.6%,RSD 2.44%.Conclusion： The method was simple and accurate,and can be used for the quality control of Tianma Zhuanggu Pills.

关 键 词：天麻壮骨丸 天麻素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]