MK-801诱发的小鼠精神分裂症样症状与脑内Parvalbumin异常表达的关系  

The relation between MK- 801 -induced schizoid symptoms and abnormal intracerebral expression of parvalbumin in mice

作　　者：马传响[1] 张静[1] 郑殿杰[2] 刘芳[1] 刘均涛[2] 宋亮[2] 陈幽婷[1] 

机构地区：[1]徐州医学院解剖学教研室,江苏徐州221004 [2]徐州医学院神经生物学教研室

出　　处：《徐州医学院学报》2011年第5期310-314,共5页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：徐州市科技计划项目（XM07C057）

摘　　要：目的 以中等剂量的MK-801腹腔注射制作小鼠精神分裂症样模型,检测小鼠脑内钙结合蛋白Parvalbumin（小白蛋白,PV）的异常表达部位,探讨与已知精神分裂症相关脑区的关系,为深入研究精神分裂症的发病机制提供形态学和神经化学依据.方法 雄性成年昆明小鼠,随机分为生理盐水组和MK-801组.用Sams-Dodd刻板行为评分和一次性被动回避反应（OPAR）检测小鼠的行为学改变;用免疫组织化学染色法检测各组小鼠脑内PV蛋白的表达情况.结果 ① MK-801组小鼠刻板行为评分明显增高（P＜0.01）,记忆能力显著下降（P＜0.01）,符合精神分裂症样模型小鼠的表现.②MK-801组小鼠在前额皮质、海马、纹状体、杏仁核及丘脑网状核等脑区PV蛋白的表达明显减少.结论 隶属边缘系统的前额皮质、海马、纹状体、杏仁核及丘脑网状核等脑区内的PV阳性神经元数量的减少可能与精神分裂症的病理生理学机制密切相关.Objective To detect the regions of abnormal parvalbumin （PV） expression in the mouse brain with the establishment of animal model of mouse schizoid symptoms by the intraperitoneal administration of medium dose of MK - 801, to investigate the correlation with the known brain area connected with schizophrenia, and to provide evidence of morphology and neurochemistry for thorough research of nosopoietic mechanism of schizophrenia. Methods Adult male Kunming mice were randomized into normal saline group and MK- 801 group （0.6 mg/kg）, and their ethological changes were determined by Sams - Dodd Stereotyped Behavior Scores and Once Passive Avoidance Response （ OPAR）, respectively. Meanwhile, immunohistochemical method was employed to detect and evaluate the PV expression in mice. Resuits 1. Compared with the normal saline group, the Stereotyped Behavior score of mice in the MK - 801 group significantly increased （P 〈 0. 01 ） and the ability of memory markedly descended （P 〈 0.01 ）, which was consistent with the schizoid symptoms model of mouse performance. 2. Compared with the normal saline group, a significant reduction of PV expression in the MK- 801 groups was observed in the mouse encephalie regions： prefrontal cortex （mPFc）, hippocampus （Hi） , corpus striatum （CS） , amygdaloid nucleus （Am） and reticular thalamic nucleus （Rt） , etc. Conclusion The significant reduction in the quantity of PV - positive neurons of such limbic system attached to mouse brain as mPFc, Hi, CS, Am and Rt, etc. may be closely correlated with pathophysiological mechanisms of the schizophrenia.

关 键 词：精神分裂症 MK-801 PARVALBUMIN 中间神经元 

分 类 号：R749.38[医药卫生—神经病学与精神病学]