糖链切除对IgG1型单抗结构及功能的影响  

Effect of theoligosaccharides IgG1 on its structure and function

作　　者：陶磊[1] 饶春明[1] 高凯[1] 史新昌[1] 赵阳[1] 王军志[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2011年第11期2095-2098,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家863计划项目(2007AA021601);重大新药创制(2009ZX09307)

摘　　要：目的:分析IgG1型单抗中糖链切除对其结构与功能的影响。方法:分别测定糖链切除前后该抗体的圆二色谱、抗原结合能力以及体外补体依赖的细胞毒(CDC)活性,分析糖链切除对该单抗结构与功能的影响。结果:糖链切除后抗体的圆二色谱发生改变,抗原结合能力下降,体外CDC活性基本消失。结论:糖链是维持该IgG1型单抗正常的结构和功能的重要组成部分。Objective： To analyze effect of oligosaccharides in the Fe domain of IgG1 molecules on its structure and function. Methods：The circular dichroism spectra, （CD） ;antigen binding capability and complement dependent cytotoricity（ CDC ）activity in vitro of Abs with and without oligosaccharides were measured, through which the effect of oligosaccharides can be determined. Results：Noticeable differences in circular dichroism spectra, decreased CD20 binding capability and disappeared CDC activity in vitro were found after the removal of oligosaccharides. Conclusion： Oligosaccharides are indispensable parts of the IgG1 for maintaining its normal structure and function.

关 键 词：生物技术药物 单克隆抗体 糖链 结构 功能 圆二色谱 抗原结合力 细胞毒性 

分 类 号：R917[医药卫生—药物分析学]