β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ的工艺研究  

Preparation Technology of Baohuoside Ⅰ by Enzymolysis of Icariin with β-glucosidase

作　　者：张振海[1] 陈玲玲[1] 贾晓斌[1] 贺俊杰[1] 金鑫[1] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,南京市210028

出　　处：《中国药房》2011年第43期4059-4061,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30973944)

摘　　要：目的:研究用β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ的工艺。方法:采用β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ,以转化率为指标,通过单因素试验考察温度、pH值、酶与底物质量比、底物浓度和反应时间对转化率的影响。结果:酶解反应的优化条件为温度50℃、pH=5.0的枸橼酸-枸橼酸三钠缓冲液、酶与底物质量比1:2、底物浓度1mg·mL-1、反应时间90min。结论:β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ,转化率高,工艺简单、可靠,反应条件温和。OBJECTIVE： To study the preparation technology of baohuoside Ⅰ by enzymolysis of icariin with β-glucosidase. METHODS： Taking bioconversion rate as index,β-glucosidase was used for enzymolysis of icariin and the effects of temperature, pH value, the ratio of enzyme to substrate, substrate concentration and reaction time on conversion rate of baohuoside Ⅰ were investigated by single factor test. RESULTS： The optimum reaction conditions were determined as follows： 50 ℃, citric acid-citric acid sodium acetate buffer （pH 5.0）, the ratio of enzyme to substrate 1：2, substrate concentration 1 mg.mL-1 and reaction time 90 min. CONCLUSION： It is simple and reliable to prepare baohuoside Ⅰ byby the enzymolysis of icariin with β-glucosidase; the conversion rate is high and the technology is moderate.

关 键 词：淫羊藿苷 宝藿苷Ⅰ Β-葡萄糖苷酶 生物转化 

分 类 号：R284[医药卫生—中药学] R283[医药卫生—中医学]