浸润性乳腺癌HER一2基因扩增与蛋白表达的关系研究  

A study on the relationship between HER-2 gene amplification and protein expression in invasivebreast cancer

作　　者：陈顺平[1] 刘伟[1] 余英豪[1] 谢飞来[1] 熊喜生[1] 陈惠春[1] 李敏[1] 

机构地区：[1]南京军区福州总医院病理科,福州350025

出　　处：《中华普通外科杂志》2011年第10期829-832,共4页Chinese Journal of General Surgery

基　　金：基金项目：卫生部科研项目资助（WKJ2007-34）01）

摘　　要：目的了解HER-2基因与蛋白在乳腺癌中表达状况，探讨HER-2基因与Ki-67、P53、ER、PR、年龄、淋巴结转移、TNM分期的相关性及其临床意义。方法收集150例浸润性乳腺癌组织石蜡标本，用荧光原位杂交（FISH）技术和免疫组化（IHC）法分别检测标本中的HER-2基因及HER．2、Ki-67、1＇53、ER、PR蛋白表达情况。结果150例浸润性乳腺癌标本HER-2基因扩增率为32．68％（49／150）；其中，在HER-2蛋白（＋＋＋）的病例中HER-2基因扩增率为90．32％（28／31）；HER-2蛋白（＋＋）的病例中扩增率34．78％（16／46）；HER-2蛋白（＋）和（-）的扩增率分别为11．54％（3／26）、4．25％（2／47）。HER-2基因与ER、PR表达及TNM分期相关，而与年龄、Ki-67、P53、淋巴结转移无关。结论FISH技术和IHC技术检测HER-2基因和蛋白表达在（＋＋＋）和（-）的病例中一致性较好，IHC可作为替代FISH检测HER-2基因状态的方法。Objective To study the amplification and expression of HER-2 gene and protein in breast cancer and to investigate the relationship between HER-2 gene and Ki-67 ,P53,ER,PR,lymph node metastasis and TNM staging. Methods Fluorescence in situ hybridization （ FISH ） and immunohistochemistry（IHC） method were used to detect the amplification and expression of HER-2 and the expression of Ki-67, P53, ER, PR in 150 cases of breast cancer. Results 49 out of 150 cases were amplificated,positive rate of HER-2 gene was 32. 68%. In the 32 patients whose expression of HER-2 protein was （ ＋ ＋ ＋ ） , HER-2 gene was expressed in 28 cases. In The 46 patients whose expression of HER-2 protein was （ ＋ ＋ ）, HER-2 was expressed in 16 cases. In the 26 patients whose expression of HER-2 protein was （ ＋ ）, HER-2 was expressed in 3 cases. In the 47 cases that the expression of HER-2 protein was negative, there were 45 cases that the expression HER-2 gene was negative. The expression of HER-2 was correlated with ER, PR expression and TNM staging, but not significantly related with age, Ki-67,1）53 and lymph node metastasis. Conclusions FISH and IHC correlate well with each other in the determination of HER-2 gene amplication and protein expression in cases of invasive breast cancer.

关 键 词：乳腺肿瘤 基因 ERBB-2 原位杂交 荧光 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]