血管紧张素Ⅱ、血管紧张素（1—7）对NIT-1细胞胰岛素信号通路的影响  

Effect of angiotensin 11, angiotensin-（ 1-7 ） on insulin signaling pathway in NIT-1 cell line

作　　者：柴佳妮[1] 徐明彤[1] 薛声能[1] 唐菊英[1] 姜力丹[1] 何爽[1] 李焱[1] 严励[1] 

机构地区：[1]中山大学孙逸仙纪念医院内分泌科,广州510120

出　　处：《中华内分泌代谢杂志》2011年第10期844-848,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：广东省科技计划项目（20098030801017）

摘　　要：目的研究血管紧张素Ⅱ、血管紧张素（1-7）对胰岛β细胞胰岛素信号通路的影响。方法小鼠胰岛β细胞株NIT-1予（1）0、10^-7、10^-6、10^-5和10^-4 mol／L浓度血管紧张素Ⅱ处理24h；（2）0、10^-7、10^-6、10^-5和10^-4 mol／L浓度血管紧张素（1-7）处理24h；（3）血管紧张素Ⅱ、血管紧张素（1-7）联合处理24h，分为对照、10^-5 mol／L血管紧张素Ⅱ、10^-6 mol／L血管紧张素（1-7）、10^-5 mol／L血管紧张素Ⅱ＋10^-6 mol／L血管紧张素（1-7）组。Western印迹检测胰岛素受体β亚基酪氨酸磷酸化（IR-β-Tyr）及蛋白激酶B丝氨酸磷酸化（Akt-Ser）水平。结果血管紧张素Ⅱ浓度10^-5和10^-4 mol／L时，胰岛素刺激的IR-β_Tyr、Akt_ser表达显著降低；不同浓度血管紧张素（1-7）作用下，胰岛素刺激的IR-β-Tyr、Akt-Ser表达与对照组相比无差异；加入血管紧张素（1-7）共同孵育可逆转血管紧张素Ⅱ对Akt-Ser表达的抑制，而血管紧张素Ⅱ对IR-β-Tyr表达的抑制无效应。结论在β细胞中，血管紧张素Ⅱ抑制胰岛素信号传导，血管紧张素（1-7）可拮抗血管紧张素Ⅱ对胰岛素刺激的Akt-Ser的抑制。Objective To evaluate the effect of angiotensin Ⅱ （ Ang Ⅱ ） , angiotensin- （ 1-7 ） [ Ang- （ 1-7 ） ] , and co-action of Ang Ⅱ and Ang-（ 1-7 ） on β cell insulin signaling pathway. Methods Mouse pancreatic β cell line NIT-1 was incubated with （ 1 ） 0, 10^-7, 10^-6, 10^-5, 10^-4 mol/L concentrations of Ang Ⅱ for 24 h ; （ 2 ） 0, 10^-7, 10^-6, 10^-5, 10^-4 mol/L concentrations of Ang- （ 1-7 ） for 24 h ; （ 3 ） co-administration of Ang Ⅱ and Ang-（ 1-7 ） was divided into control, 10^-5 mol/L Ang Ⅱ, 10^-6 mol/L Ang-（ 1-7 ）, 10^-5 mol/L Ang Ⅱ ＋ 10 ^-6 mol/L Ang-（ 1-7 ） group. Tyrosine phosphorylation of insulin receptor β subunit （IR-β-Tyr）and serine phophorylation of protein kinase B（Akt-Ser） were detected by Western blot. Results Insulin-stimulated IR-β-Tyr and Akt-Ser phosphorylation was significantly decreased in Ang Ⅱ 10^-5 and 10^-4 mol/L group; no significant changes in insulin-stimulated IR-β-Tyr and Akt-Ser phosphorylation were detected between Ang-（1-7） treatment groups and control; Ang-（ 1-7 ）blocked the inhibitory effect of Ang Ⅱ on Akt-Ser phosphorylation, yet exerted no effect on Ang Ⅱ-induced IR-β-Tyr phosphorylation inhibition. Conclusion Ang Ⅱ significantly inhibits insulin signaling pathway in β cell; Ang-（ 1-7 ） reverts the inhibitory effect of Ang Ⅱ on insulin-stimulated Akt-Ser phosphorylation in β cell.

关 键 词：血管紧张素Ⅱ 血管紧张素(1-7) 胰岛Β细胞 胰岛素信号通路 

分 类 号：R363[医药卫生—病理学]