尿酸对阿尔茨海默病大鼠学习记忆能力的影响及其机制  

The effects of uric acid on learning-memory ability in the rat model of Alzheimer disease

作　　者：宋彦[1] 索爱琴[2] 丁旭萌[1] 王天虎[1] 杨国东[1] 贾兰梅[1] 

机构地区：[1]河南省南阳市第二人民医院神经内科,南阳473012 [2]河南省人民医院神经内科,郑州450014

出　　处：《中国神经精神疾病杂志》2011年第10期603-606,共4页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的观察尿酸对阿尔茨海默病(Alzheimer disease,AD)大鼠学习记忆能力的影响,并探讨作用机制。方法 Morris水迷宫实验筛选出90只大鼠,随机分为空白对照组,模型对照组,尿酸处理组(1～4组),每组15只。Aβ1-42海马注射制作AD模型,用Morris水迷宫实验观察干预前后大鼠空间学习记忆能力的变化,丙二醛(malondialdehyde,MDA)试剂盒测定海马区MDA的含量;免疫组化染色测定半胱氨酸天冬氨酸蛋白酶-3(caspase-3)的表达。结果与空白对照组比较,模型对照组大鼠空间学习记忆能力下降(P<0.01),海马区MDA含量和caspase-3明显增高(P<0.01);与模型对照组比较,2种剂量尿酸处理组大鼠的学习记忆能力明显改善(P<0.01),海马区MDA和caspase-3的表达减少(P<0.01)。结论尿酸具有改善AD大鼠空间学习记忆作用,可能与抗氧化应激作用及抑制海马中caspase-3表达有关。Objective To investigate the effects of uric acid on learning-memory ability in the rat model of Alzheimer disease induced by Aβ1-42. Methods SD rats were divided into blank control group, AD group, uric acid treatment group （group 1-4）（n = 15）. AD animal model was established by injecting Aβ1-42 into the hippocampus; In the first 1-21 day, uric acid group 1,2,3 and 4 were intraperitoneally injected with 1,5,10,20 mg/（kg·d） uric acid, respectively. The blank control group and AD group were intraperitoneally injected with equal dose of saline. All animals were evaluated using Morris water-maze test. Malondialdehyde （MDA） content in _the hippocampus was measured by using MDA reagent box and Caspase-3-positive neurons in the hippocampus were identified by using immunohistochem- istry. Results Compared with the blank control group, the learning-memory ability was significantly declined and MDA content and the expression of Caspase-3-positive cell in the hippocampus was significantly increased at AD group, uric acid group 1, 2, 3 and 4（P 〈 0.01）. The improvements in learning-memory, the reduced MDA content and the less caspase-3 expression were observed in rats treated with two doses of uric acid （P 〈 0.01 ）. Conclusions Appropriate doses of uric acid can improve the learning-memory ability by reducing the oxidative stress and inhibiting the expression of easpase-3 in the hippocampus in the rat model of Alzheimer disease.

关 键 词：尿酸 阿尔茨海默病 学习记忆 丙二醛 半胱氨酸天冬氨酸蛋白酶-3 

分 类 号：R749.16[医药卫生—神经病学与精神病学]