RGD偶联吉西他滨白蛋白纳米粒对胰腺癌细胞增殖抑制作用的研究  

Study on inhibitory effects of RGD-conjugated gemcitabine-loaded albumin nanoparticles on the proliferation of BxPC-3 cells in vitro

作　　者：吉顺荣 吴闻哲[3] 王浩[3] 张波 刘辰[1,2] 龙江 虞先濬 倪泉兴 徐近[1,2] 

机构地区：[1]复旦大学附属肿瘤医院胰腺肝胆外科 [2]复旦大学上海医学院肿瘤学系,上海200032 [3]药物制剂国家工程研究中心,上海201203

出　　处：《外科理论与实践》2011年第5期470-473,共4页Journal of Surgery Concepts & Practice

基　　金：上海市经委引进技术消化吸收再创新项目(07XI-028)

摘　　要：目的:通过体外试验,探讨RGD偶联吉西他滨白蛋白纳米粒(RGD-BSANP-GEM)对胰腺癌细胞株BxPC-3的体外增殖的抑制作用。方法:以人胰腺癌细胞株BxPC-3为研究对象,分为5个给药组:BSANP对照组、RGD-BSANP组、BSANP-GEM组、GEM原药组、RGD-BSANP-GEM组。运用MTT法检测给药后24 h、48 h和72 h的增殖抑制率。结果:BSANP-GEM组细胞抑制率高于GEM组(P<0.05),RGD-BSANP-GEM组细胞抑制率高于BSANP-GEM组。同时RGD-BSANP-GEM组对胰腺癌细胞株的抑制率与给药浓度和作用时间呈正相关。结论:体外RGD环肽能够增加BSANP-GEM对胰腺癌细胞的增殖抑制作用。Objective To explore the inhibitory effects of RGD-conjugated gemcitabine（GEM）-loaded albumin nanoparticles（ANP） on the proliferation of BxPC-3 cells in vitro.Methods Human pancreatic carcinoma BxPC-3 cells were cultured and then treated 5 different combinations of drugs（BSANP controls,RGD-BSANP,BSANP-GEM,GEM and RGD-BSANP-GEM） respectively.The inhibition rate of BxPC-3 cells was detected by MTT.Results The gemcitabine-loaded albumin nanoparticles produced higher inhibitory effects than gemcitabine alone.The RGD-conjugated gemcitabine-loaded albumin nanoparticles produced even higher anti-proliferation rate than those without RGD conjugation.Conclusions RGD peptides can increase the anti-proliferation rate of gemcitabine-loaded albumin nanoparticles on BxPC-3 cells in vitro.

关 键 词：RGD多肽 吉西他滨 白蛋白纳米粒 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]