5-Aza-CdR对食管鳞癌甲基化基因的影响  

Effect of 5-Aza-CdR on methylation gene in esophageal squamous cell carcinoma

作　　者：耿月华[1] 秦旭[1] 刘清[1] 郑树涛[1] 刘涛[1] 林仁勇[1] 卢晓梅[1] 吕国栋[1] 

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054

出　　处：《疾病预防控制通报》2011年第5期5-9,共5页Bulletin of Disease Control & Prevention(China)

基　　金：国家自然科学基金项目(30860097);新疆医科大学科研奖励基金(2011YFY17)

摘　　要：目的探讨5-aza-CdR对食管鳞癌甲基化基因的影响。方法应用人类全基因组寡核苷酸微阵列芯片,荧光双交换法检测5-aza-CdR干预的食管鳞癌细胞Eca-109与正常培养的Eca-109细胞中的差异表达基因,并进行生物信息学分析。结果经统计学分析,共筛选获得384个差异表达基因,其中303个基因表达上调,81个基因表达下调;差异基因的功能涉及信号转导、物质合成代谢、细胞周期、细胞增殖、细胞凋亡、DNA转录、DNA复制、DNA修复、氧化还原、物质运输、免疫反应等;上调表达基因中有138个基因分别含有1～5个CpG岛序列,占总上调基因的45.54%。结论5-aza-CdR可以影响食管鳞癌细胞中基因异常的甲基化修饰,调控肿瘤细胞的异常凋亡和分化,为进一步研究食管鳞癌致病分子机制,提供表观遗传学的依据。Objective To explore molecular mechanism on methylation in esophageal squamous cell carcinoma.Methods With fluorescent dye swap method,the different genes between Eca-109 treated by 5-aza-CdR for 96 h and control group were tested by human genome oligonucleotide microarrays.Results The microarray analysis showed that 384 genes were differentially expressed between Eca-109 treated by 5-aza-CdR for 96 h and control group,303 genes were up-regulated and 81 genes were down-regulated.The function of differential expression genes were involved in signal transduction,material metabolism,cell cycle,cell proliferation,apoptosis,DNA transcription,DNA replication,DNA repair,oxidant response,material transport and immune response.There were 138 genes with 1-5 CpG island sequences in up-regulated genes,which accounted for 45.54% among total up-regulated genes.Conclusions 5-aza-CdR can reverse the methylation modification of many genes in esophageal squamous cell carcinoma,and promote tumor cell apoptosis and differentiation,which provides the foundation for the study on abnormal methylation mechanism in esophageal squamous cell carcinoma.

关 键 词：食管鳞癌 Eca-109细胞系 5-杂氮-2’-脱氧胞苷 基因芯片 

分 类 号：R735.1[医药卫生—肿瘤] Q343.1[医药卫生—临床医学]