高尿酸血症肾病模型大鼠TGF-β1、TNF-α变化及中药的调节作用  

Study on the Changes of TGF-β1 and TNF-α in Rat Model With Hyperuricemic Nephropathy and the Adjustment Effects of Chinese Herb

作　　者：叶明伟 陈翔 杨成晓[2] 张景红[2] 郑士荣[2] 

机构地区：[1]浙江省三门县人民医院,浙江台州317100 [2]解放军第85医院,上海200052

出　　处：《深圳中西医结合杂志》2011年第5期268-271,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：上海市医学发展基金(05Ⅱ010)

摘　　要：目的:观察高尿酸肾病模型大鼠血尿酸、肾脏组织病理学及肾组织中的TGF-β1、TNF-α的变化及中药复方对TGF-β1、TNF-α表达的调节作用。方法:采用高嘌呤饲料加腺嘌呤灌胃制作高尿酸血症肾病大鼠模型,并分组处理,中药治疗组用中药煎剂,别嘌呤醇治疗组用别嘌呤醇溶液,模型对照组及正常对照组给予蒸馏水灌胃,用药持续4周。观察血尿酸、肾脏组织病理学及肾组织中的TGF-β1、TNF-α的变化。同时,观察中药复方对上述各项指标的调节作用。结果:高尿酸血症肾病大鼠肾组织TGF-β1、TNF-α表达明显上调,且中药复方能调节其表达。结论:高尿酸血症肾病大鼠肾组织TGF-β1、TNF-α表达明显上调;中药有调节高尿酸血症肾病大鼠肾组织中TGF-β1、TNF-α表达的作用。Objective To observe the blood uric acid level,the changes of pathology and expressions of TGF-β1 and TNF-α in the kidney tissue of the rat model with hyperuricemic nephropathy,and explore the adjustment effects of Chinese herb on the expressions of TGF-β1 and TNF-α.Methods The rats were fed with high-purine diet and treated with adenine by intragastric administration to establish the rat model with hyperuricemic nephropathy.All rats were randomized into 4 groups：three model groups were treated with Chinese herb solution,allopurinol and distilled water respectively;and one normal control group was treated with distilled water.The drug intervention lasted 4 weeks.The blood acid uric level of the rat model with hyperuricemic nephropathy was measured,the changes of pathology and expressions of TGF-β1 and TNF-α in the kidney tissue were observed.And the adjustment effects of Chinese herb on the above indexes were also investigated.Results The blood uric acid level and the expression level of TGF-β1 and TNF-α in kidney tissue of the rat model with hyperuricemic nephropathy were increased significantly,comparing with normal control group（P 〈 0.01）.Comparing with model group,the blood uric acid level and the expression level of TGF-β1 and TNF-α in kidney tissue of Chinese herb group were decreased obviously（P 〈 0.01）.Conclusion The blood uric acid level and the expression level of TGF-β1 and TNF-α in kidney tissue of the rat model with hyperuricemic nephropathy were improved significantly,and the Chinese herb have the adjustment effects on the expressions of TGF-β1 and TNF-α in kidney tissue of the rat model with hyperuricemic nephropathy.

关 键 词：高尿酸血症肾病模型 TGF-Β1 TNF-Α 中药复方 

分 类 号：R-332[医药卫生] R589.7