小凹蛋白-1在食管鳞癌中的表达及其意义  

Significance of caveolin-1 expression in esophageal squamous cell carcinoma

作　　者：高万峰[1] 杨光[1] 史昌乾 叶圣权 丁国臣[1] 于兰[2] 韩忠学[2] 刘静[2] 

机构地区：[1]武警辽宁总队医院医务处,沈阳110034 [2]武警辽宁总队医院检验科,沈阳110034

出　　处：《武警医学》2011年第10期842-845,848,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的研究小凹蛋白-1(caveolin-1)在不同食管组织中的表达及其与临床生物学行为的关系,进而探讨其在食管癌发生、发展中的作用机制。方法采用免疫组织化学方法分析126例食管鳞状细胞癌、33例食管原位癌及53例癌旁正常食管黏膜组织中caveolin-1的表达情况,并根据临床完整资料评价其表达与生物学行为的关系;另收集其中30例新鲜食管癌组织及其癌旁正常食管组织,采用Western印迹法测定组织中caveolin-1的表达情况。结果 (1)caveolin-1在食管鳞癌、原位癌及癌旁正常组织中的阳性表达率分别为86.5%、51.5%和22.7%,差异均具有统计学意义(P<0.01)。(2)caveolin-1在食管鳞癌临床Ⅰ期、Ⅱ期及Ⅲ期的表达率分别为77.6%,86.1%和97.6%,癌组织的表达强度随着肿瘤临床分期的升高而逐渐增强,差异具有统计学意义(P=0.039);淋巴结转移组caveolin-1的表达明显高于无淋巴结转移组(P=0.023)。(3)Western印迹法结果进一步证实食管鳞癌组织的caveolin-1的表达明显低于癌旁正常食管组织(P<0.01)。结论提示caveolin-1的高表达可能参与了食管癌的发生、发展过程;caveolin-1的表达与食管癌临床分期有一定的关系,其很可能成为潜在的治疗靶点及判断食管癌预后的有价值的肿瘤标记物。Objective To study the expression of caveolin - 1 in esophageal squamous cell carcinoma, and its mechanism of action in the process of occurrence and development. Methods Immunohistochemistry was used to analyze the expression of caveolin - 1 in 126 patients with esophageal squamous cell carcinoma, 33 patients with esophageal carcinoma in situ, and 53 pateints with adjacent normal esophageal mucosa before the relationship between its expression and biological behavior was evaluated on the basis of complete clinical information. In addition, Western blotting was used to determine the expression of caveolin - 1 in 30 patients with esopha- geal cancer and adjacent normal esophageal tissues. Results （ 1 ） The positive rate of caveolin - 1 in esophageal squamous cell carci- noma, carcinoma in situ, and adjacent normal tissues was 86.5% , 51.5% and 22.7% , respectively. The difference between them was statistically significant （P 〈0.01 ）. （2） The positive rate of caveolin- 1 in stages Ⅰ,Ⅱ,Ⅲ of esophageal squamous cell carci- noma was 77,6% , 86.1% ,and 97.6% , respectively. The expression intensity of carcinoma tissue gradually increased with the ad- vance of clinical stages, the differences being statistically significant （ P = 0. 039 ）. The positive rate of caveolin - 1 with group of lymph node metastasis was significantly higher than those without lymph node metastasis （ P = 0. 023 ）. （ 3 ） Western blotting results further confirmed that the expression of caveolin - 1 in esophageal squamous cell carcinoma was significantly lower than that in adjacent normal esophageal tissue （ P 〈 0.01 ）. Conclusions The high expression of caveolin - 1 may be involved in the occurrence and devel- opment of esophageal cancer. The expression of caveolin - 1 is correlated with the clinical stage of esophageal cancer, and is a potential therapeutic target and a valuable prognostic indicator of esophageal cancer progression.

关 键 词：食管肿瘤 小凹蛋白-1 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]