胰腺癌组织中Smad4、上皮型钙黏蛋白及β-连环蛋白的表达及临床意义  

Expression and clinical significance of Smad4、E-cadherin and β-catenin in pancreatic cancer

作　　者：肖蔚[1] 焦霞[1] 赵燕[1] 周彤敏 窦荣荣 于鸿[1] 

机构地区：[1]泰州市人民医院病理科,江苏泰州225300

出　　处：《江苏大学学报（医学版）》2011年第5期423-427,共5页Journal of Jiangsu University：Medicine Edition

基　　金：南通大学自然科学基金资助项目(10Z088);泰州市311工程人才基金资助项目(201036)

摘　　要：目的:观察Smad4、上皮型钙黏蛋白(E-cadherin)及β-连环蛋白(β-catenin)在胰腺癌组织中的表达,探讨Smad4在胰腺癌侵袭和转移过程中的作用机制。方法:构建胰腺癌组织芯片,采用免疫组织化学方法检测Smad4、E-cadherin及β-连环蛋白表达情况,并与临床病理资料作对照分析。结果:88例胰腺导管腺癌组织标本中Smad4、E-cad-herin及β-连环蛋白阳性率分别为55.7%(49/88)、51.1%(45/88)和60.2%(53/88),明显低于正常胰腺组织中的表达水平(P<0.01)。Smad4、E-cadherin和β-连环蛋白的阳性表达与胰腺癌患者的性别、年龄、肿瘤的位置及大小均无关(P>0.05),而与胰腺癌组织病理学分级、临床TNM分期及淋巴结转移密切相关(P<0.01)。Smad4、E-cadherin和β-连环蛋白在胰腺癌组织中的蛋白表达均呈正相关(P<0.01)。Kaplan-Meier生存分析显示,胰腺癌组织中Smad4、E-cadherin及β-连环蛋白表达阴性的患者生存时间缩短。结论:Smad4、E-cadherin及β-连环蛋白在胰腺癌中表达均降低,Smad4可能通过参与胰腺癌组织内E-cadherin和β-连环蛋白的代谢过程进而影响胰腺癌的分化、浸润及转移。Objective： To elucidate the mechanism of pancreatic cancer invasion and metastasis;and the expression of Smad4,E-cadherin and β-catenin in pancreatic cancer tissue was going to observe.Methods： The expression of Smad4,E-cadherin and β-catenin protein in a constructed tissue array was analyzed by immunohistochemistry and cross referencing of clinicopathologic data.Results： In tissue samples from 88 pancreatic cancer cases,positive immunoreactivity of Smad4,E-cadherin and β-catenin expressions,which were 55.7%（49/88）、51.1%（45/88） and 60.2%（53/88）,respectively,were significantly lower than those in normal pancreatic tissue（P0.01）.There was no significant difference between positive immunoreactivity of Smad4,E-cadherin and β-catenin expression with clinicopathologic data of pancreatic cancer patients including sexuality,age,tumor site and tumor size（P0.05）.Positive immunoreactivity of Smad4,E-cadherin and β-catenin expressions were significant correlated with tumor histopathological grade,clinical TNM stages and lymphnode metastasis（P0.01）.There was statistically positive correlation among Smad4,E-cadherin and β-catenin expressions in pancreatic cancer tissue（P0.01）.There was statistically correlation between positive immunoreactivity of Smad4,E-cadherin and β-catenin expression with survival time by Kaplan-Meier analysis.Conclusion： Smad4,E-cadherin and β-catenin were lowly co-expressed in pancreatic cancer tissue.Smad4 may inhibit the progression of pancreatic cancer through reducing E-cadherin and β-catenin production in pancreatic cancer tissue.

关 键 词：胰腺癌 SMAD4 上皮型钙黏蛋白 Β-连环蛋白 

分 类 号：R735.9[医药卫生—肿瘤]