ID1对大鼠C6细胞侵袭转移能力的影响  

The effect of ID1 on invasive ability of C6

作　　者：田刚[1] 樊明新[1] 陈祖容 徐英[1] 杨江河[1] 卢宁[3] 李华[1] 张翼冠[4] 

机构地区：[1]兰州军区乌鲁木齐总医院神经外科,新疆乌鲁木齐830000 [2]福建省莆田市涵江区顶铺街366号涵江医院神经外科,福建莆田351111 [3]兰州军区乌鲁木齐总医院肿瘤科,新疆乌鲁木齐830000 [4]四川省中医药科学院,四川成都610041

出　　处：《现代生物医学进展》2011年第21期4047-4049,4053,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助(30901990)

摘　　要：目的:观察ID1对大鼠胶质瘤细胞(C6细胞)侵袭转移能力的影响。方法:以C6细胞为研究对象,ID1siRNA通过脂质体转染C6细胞,westernblot检测C6细胞中ID1、P-NF-КBp65蛋白的表达、Elisa法检测细胞培养上清MMP-9的含量,Transwell小室观察成的转移侵袭能力。结果:干扰ID1后能显著降低C6细胞中P-NF-КBp65蛋白、细胞培养上清中MMP-9的含量(P<0.01),降低C6细胞的转移侵袭能力(P<0.01)。结论:干扰ID1能抑制C6细胞的转移侵袭,其机制可能与抑制C6细胞中P-NF-КBp65蛋白、MMP-9的表达有关。Objective： To study the effect of ID 1 on invasive ability of C6. Methods： C6 cells were divide into control, non sense, ID1 siRNA groups. The effects of IDlsiRNA on C6 cells invasive ability in vitro was measured by Boyden chamber invasive models. Contents of ID1, NF-K Bp65, MMP-9 protein were detected by western blot and ELISA methods, respectively. Result： In ID1 siRNA group, contents ofNF-K Bp65 and MMP-9 protein were decreased markedly（P〈0.01）, the invasive ability was decreased markedly（P〈0. 01）, too. Conclusion： IDI could increase the invasive ability of C6 cell.

关 键 词：NF-КBp65 MMP-9 ID1 侵袭力 

分 类 号：Q95-3[生物学—动物学] R730.231[医药卫生—肿瘤]