鲍曼不动杆菌对喹诺酮类药物的耐药机制分析  

Analysis on the resistance mechanism ofAcinetobacter baumanii to quinolones

作　　者：戴宁[1] 陈济超[2] 王占伟[3] 赵素蕊[3] 高占成[4] 

机构地区：[1]北京大学人民医院呼吸与危重症医学科,北京市第六医院干部病房,北京市100007 [2]北京大学航天中心医院呼吸内科,北京市100046 [3]北京大学人民医院微生物实验室,北京市100044 [4]北京大学人民医院呼吸与危重症医学科,北京市100044

出　　处：《实用检验医师杂志》2011年第2期94-96,共3页Chinese Journal of Clinical Pathologist

摘　　要：目的 探讨鲍曼不动杆菌（Acinetobacterbaumanii，Ab）对喹诺酮类药物的耐药机制。方法收集2007年11月至2008年10月全国多省市21家医院院内下呼吸道感染住院患者痰标本2698份进行培养鉴定，对Ab分离株进行药敏检测，采用聚合酶链反应对Ab的不同耐药基因进行扩增，并进行序列分析。结果2698份痰标本共计培养出39株Ab。对环丙沙星耐药率为61．54％，对左氧氟沙星耐药率为53．85％。39株Ah中有30株（76．92％）发生parC基因突变，19株（48．72％）发生gyrA基因突变，15株同时发生parC基因和gyrA基因突变。结论Ab对喹诺酮类药物耐药机制主要与gyrA、parC基因突变有关。Objective To explore the drug resistance mechanism ofAcinetobacter baumanti （Ab） to quinolones. Methods 2698 sputum specimens were collected during Nov. 2007 to Oct. 2008 from patients with lower respiratory infection in 21 hospitals. All specimens were cultivated and the isolated Ab strains were identified. Drug sensitivity assay of these strains were performed. Drug resistance genes of Ab were amplified by PCR and the gene sequence were analyzed. Results 39 strains of Ab were identified from 2698 sputum specimens. 61.54% of the bacteria were resistant to ciprofloxaein and 53.85% were resistant to levofloxacin. There were 30 strains（76.92%） had parC gene mutation and 19 strains（48.72%） had gyrA gene mutation and 15 strains both had parC gene and gyrA gene mutation. Conclusion The mechanism of Ab drug resistance to quinolones are closely associated with parC and gyrA gene mutation.

关 键 词：鲍曼不动杆菌 喹诺酮类 抗药性 GYRA基因 PARC基因 

分 类 号：R978.19[医药卫生—药品]