康复训练对脑出血大鼠神经细胞再生的影响  

Effects of rehabilitation on the regeneration of nerve cells after experimental cerebral hemorrhage in rats

作　　者：刘珍[1] 武衡[1] 谢明[1] 

机构地区：[1]南华大学附属第一医院神经内科 ,衡阳421001

出　　处：《中华物理医学与康复杂志》2011年第10期731-735,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖南省教育厅基金（06C702）

摘　　要：目的探讨康复训练对大鼠脑出血后神经细胞再生的影响。方法将75只SD大鼠分为康复组、制动组和假手术组，每组25只。康复组和制动组应用胶原酶Ⅶ注射人苍白球诱导脑出血模型，假手术组用生理盐水替代胶原酶。康复组每天予以抓握、平衡、旋转等训练，制动组置于网状笼内固定。各组分成脑出血后第1，4，7，14，28天共5个时间点，每个时间点5只。对不同组大鼠进行神经功能评分，用5-溴脱氧尿核苷（BrdU）标记S期增殖细胞；BrdU免疫组化单标、BrdU／微管相关蛋白（MAP）和BrdU／神经元特异性核蛋白（NeuN）免疫荧光双标法检测大鼠侧脑室下区（SVZ）和齿状回颗粒下区（SGZ）细胞的增殖、迁移与分化。结果康复组神经功能评分明显低于制动组（P〈0．05）；制动组大鼠SVZ的BrdU＋细胞在各时间点上少于康复组大鼠；脑出血后第7天，康复组SVZ区BrdU＋细胞表达显著增加且高于制动组（P〈0．05），14d时SVZ区BrdU＋细胞表达减少，同时在出血侧纹状体发现BrdU＋／MAP＋细胞，是制动组的1．8倍（P〈0．05）；脑出血后第28天，康复组出血侧纹状体神经元分化比率是制动组的2．3倍（P〈0．05）。结论康复训练可促进脑出血后神经细胞再生。Objective To study the effects of rehabilitation training on the regeneration of nerve cells in rats after intracerebral hemorrhage （ICH）. Methods A total of 75 male SD rats were randomized into a training group, a control group and a sham operated group, 25 rats/group. The ICH models were induced by stereotactical injection of collagenase type VII into the globus pallidus. The training group was trained with grasp, balancing and rotating exercise every day, the control group was restricted to their cages, and the sham operated group received normal saline injections. Each group was further subdivided into 1, 4, 7, 14 and 28 day subgroups. Neurological function was measured in each group. Bromodeoxyuridine （BrdU） was used to label S phase cells, immunohistochemical single and double staining with antibodies against BrdU, microtubal-associated protein （MAP） and neuronal nuclei （NeuN） were used to determine neuronal proliferation, migration and differentiation in the subventricular zone （SVZ） and subgranular zone （SGZ） in the training and control groups. Results The motor function scores of the animals in the rehabilitation group were significantly lower than those of the control group. Proliferative BrdU ＋ cells of the SVZ and SGZ in the control group rats were clearly less than those in the rehabilitation training rats at all time points. The results of the immunohistochemical double staining indicated that one week after ICH BrdU ＋/MAP ＋ cells in the SVZ had increased significantly in the training group compared to the control group, and then decreased two weeks later. At the same time, BrdU＋/MAP cells were found in the striatal boundary on the hemorrhage side, in numbers up to 8 times that in the control group. In the rehabilitation group striatal neuron differentiation on the hemorrhage side was 2 to 3 times that in the control group. Conclusion Rehabilitative training can enhance nerve cell proliferation, regeneration and neuron migration after ICH.

关 键 词：康复训练 脑出血 5-溴脱氧尿核苷 微管相关蛋白 神经元特异性核蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]