M2-PK和HSP90α在胃癌患者血清中的表达及意义  

Clinical significance of serum levels of tumor type M2-pyruvate kinase and heat shock protein 90αin patients with gastric cancer

作　　者：刘志刚 宋永春[2] 孙瑞芳[3] 雷光焰 

机构地区：[1]陕西省肿瘤医院胸部肿瘤外科,陕西省西安市710061 [2]西安交通大学医学院第一附属医院肿瘤外科,陕西省西安市710061 [3]西安交通大学医学院,陕西省西安市710061

出　　处：《世界华人消化杂志》2011年第25期2673-2678,共6页World Chinese Journal of Digestology

基　　金：Correspondence to:Guang-Yan Lei,Department of Chest Surgery,Tumor Hospital of Shannxi Province,Xi'an 710061,Shaanxi Province,[e-mail]

摘　　要：目的:检测肿瘤型M2丙酮酸激酶(M2-PK)与热休克蛋白90α(HSP90α)在胃癌患者血清中的表达水平,并与癌胚抗原(CEA)进行比较,评估其临床应用价值.方法:收集胃癌患者和健康对照血清标本各80例,检测血清中的M2-PK、HSP90α及CEA的含量.结果:M2-PK、HSP90α和CEA在胃癌患者血清的表达水平均显著高于对照组(P<0.05).M2-PK的灵敏度为56.25%,特异度为91.25%.HSP90α的灵敏度为52.50%,特异度为92.50%.CEA灵敏度为45.00%,特异度为92.50%.与HSP90α和CEA相比,M2-PK灵敏度较高,三者联合检测的灵敏度高达91.25%.胃癌组间比较结果显示,M2-PK在病变大于5cm、临床病理晚期、肿瘤浸润深度较深和存在淋巴结转移的情况表达水平较高,有显著性差异(P<0.05).此外,Spearman相关分析显示,M2-PK和HSP90α的在血清中的表达水平具有明显的相关性.结论:M2-PK和HSP90α可以作为潜在的胃癌血清标志物,且M2-PK和HSP90α可能存在较强的正相关关系,可能对胃癌的诊断、治疗和预后具有较高的应用价值.AIM： To measure serum levels of tumor type M2 pyruvate kinase （M2-PK） and heat shock protein 90α （HSP90α） in patients with gastric cancer and to evaluate their clinical significance by comparing to serum carcinoembryonic antigen （CEA）.METHODS： A total of 80 patients with gastric cancer and 80 controls were enrolled in this study. Serum levels of M2-PK and HSP90α were measured by ELISA, while those of CEA were determined by radioimmunoassay.RESULTS： Serum levels of M2-PK, HSP90α and CEA were significantly higher in patients with gastric cancer than in controls （all P 〈 0.05）. The sensitivity and specificity of detection of M2- PK, HSP900α and CEA were 56.25% and 91.25%, 52.50% and 92.50%, and 45.00% and 92.50% respectively. Compared to detection of HSP90α and CEA alone, detection of M2-PK had a higher sensitivity. The sensitivity of combined detection of M2-PK, HSP90a and CEA was increased to 91.25%. Serum M2-PK was positively correlated with tumor size, TNM stage, invasion depth and lymph node metastasis （all P 〈 0.05）. A significant positive correlation was noted between serum M2-PK and HSP90α levels in patients with gastric cancer.CONCLUSION： Serum M2-PK and HSPgOa can be used as potential markers for gastric cancer. There is a strong positive correlation between serum M2-PK and HSP90a levels in patients with gastric cancer.

关 键 词：肿瘤型丙酮酸激酶 热休克蛋白90Α 胃 癌 标志物 

分 类 号：R735.2[医药卫生—肿瘤]