比格犬骨髓基质细胞和牙周韧带成纤维细胞体外再生组织能力的比较  

Comparison of Regenerative Tissues Potentials between BMSCs and PDLFs in Beagle Dog

作　　者：林敏魁[1] 黄永玲[1] 钟泉[1] 江俊[1] 赵欣[1] 闫福华[1] 

机构地区：[1]福建医科大学口腔医学院福建省高校重点口腔医学实验室,福州350002

出　　处：《福建医科大学学报》2011年第4期242-245,共4页Journal of Fujian Medical University

基　　金：国家自然科学基金(30471892);福建省教育厅资助省属高校项目(2008F5023);福建省自然科学基金面上项目(2011J01166)

摘　　要：目的通过比较比格犬牙周韧带成纤维细胞(PDLFs)和骨髓基质细胞(BMSCs)体外促进牙周相关组织再生潜能,为牙周组织的再生治疗提供依据。方法通过体外ELISA、免疫细胞化学染色、Von Kossa染色分别比较BMSCs和PDLFs这两种细胞的碱性磷酸酶(ALP)、骨保护素(OPG)和胶原Ⅻ、形成的钙化结节,用实时定量PCR比较这两种细胞的OPG和胶原Ⅻ基因的表达。结果 Realtime PCR检测和免疫细胞化学染色结果均发现PDLFs中胶原Ⅻ表达量高于BMSCs(P<0.05);而ALP活性检测、OPG染色及Von Kossa染色等结果都表明其成骨能力低于BMSCs(P<0.05)。结论 PDLFs体外成骨能力低于BMSCs,但有较高的牙周韧带形成潜力。Objective To compare the ability of periodontal tissue regeneration between periodontal ligament fibroblasts（PDLFs） and bone marrow stromal cells（BMSCs）,to provide foundation for periodontal tissue regeneration.Methods Immunocytochemistry,Von Kossa,Realtime PCR and some other ways were employed to compare the ability of tissue regeneration between PDLFs and BMSCs in vitro.Results Data of Realtime PCR and immunocytochemistry showed that the levels of collagen Ⅻ in PDLFs were higher than in BMSCs（P0.05）.But the expression of OPG,the activity of ALP and the result of Von Kossa showed that the osteogenic potential of BMSCs was higher than PDLFs.Conclusions It was showed that the osteogenic potential of BMSCs was higher than PDLFs,while the ability of forming periodontal ligament was lower than PDLFs in vitro.

关 键 词：骨髓细胞 成纤维细胞 组织再生 韧带 牙周组织 引导组织再生 牙同 聚合酶链反应 疾病模型 动物 

分 类 号：R781.4[医药卫生—口腔医学]