牙龈卟啉单胞菌脂多糖对牙周韧带细胞在牙骨质片附着和增殖的影响  

Effect of Pg-LPS on Attachment and Proliferation of PDLC on the Slices of Cement

作　　者：孔宁静[1] 李艳芬[2] 闫福华[2] 刘建国[1] 李厚轩[2] 赵欣[2] 

机构地区：[1]遵义医学院口腔医学院,遵义563000 [2]福建医科大学口腔医学院福建省高校口腔医学重点实验室,福州350002

出　　处：《福建医科大学学报》2011年第4期251-253,共3页Journal of Fujian Medical University

基　　金：福建医科大学苗圃科研基金(2010MP016);福建医科大学附属口腔医院重点学科建设学术发展基金(闽医大口腔[2008]39号);福建省教育厅资助省属高校项目(2008F5023)

摘　　要：目的研究牙骨质片经不同浓度牙龈卟啉单胞菌脂多糖(Pg-LPS)处理后对牙周韧带细胞(PDLC)附着和增殖的影响。方法用含不同浓度(0,1,10)μg/mL的Pg-LPS培养液培养PDLC于牙骨质片,24、72h后用MTT法检测PDLC在牙骨质片上的附着、增殖的细胞数量,同时行扫描电镜观察。结果高浓度10μg/mLPg-LPS组PDLC细胞数量明显低于空白对照组(P<0.05),而1μg/mL Pg-LPS组PDLC细胞数量与空白对照组差别无统计学意义(P>0.05)。结论低浓度Pg-LPS不会影响PDLC在牙骨质片上的附着及增殖。Objective To observe the effects of different concentration of LPS on attachment and proliferation of PDLC on the slices of cement.Methods PDLC were seeded on the slices of cement in the presence of LPS（0,1 or 10 μg/mL）,PDLC attachment and proliferation were detected by MTT assay on 1 d and 3 d.Results The number of cells attached and proliferated on the slices of cement incubated with 10 μg/mL Pg-LPS were deceased in contrast to control groups（P0.05）.Conclusion LPS with a concentration of lower than 1 μg/mL did not affect the attachment and proliferation of PDLC on slice of cement.

关 键 词：脂多糖类 卟啉单胞菌 牙髓 韧带 牙周膜 牙骨质 细胞增殖 

分 类 号：R780.2[医药卫生—口腔医学]