外周血中生存素的表达与结肠癌预后的相关研究  

Survivin expression in peripheral blood of patients with colon cancer and its relation to prognosis

作　　者：刘金龙[1] 李森龙[1] 李雪[1] 张学军[1] 孙志德[1] 

机构地区：[1]承德医学院附属医院普外一科,河北承德067000

出　　处：《中国普通外科杂志》2011年第10期1067-1070,共4页China Journal of General Surgery

摘　　要：目的探讨检测外周血生存素(survivin)的表达与结肠癌复发、转移的关系。方法采用反转录-聚合酶链反应-酶联免疫吸附试验(RT-PCR-ELISA)方法检测结肠癌患者外周血中survivinmRNA的表达,分析其与临床病理因素之间的关系。对其中51例患者进行36个月随访,比较复发及转移时间与外周血中survivin mRNA表达之间的关系。结果 95例结肠癌患者中35例survivin mRNA表达阳性(36.8%)。肿瘤高中分化及低未分化患者survivin mRNA表达率分别为31.0%和45.0%(P<0.05),浸润深度达浆膜层者表达率为44.0%,高于侵及黏膜下层及肌层者(20.0%)(P<0.05),淋巴结有转移者表达率为47.5%,高于无淋巴结转移者(19.4%)(P<0.05)。随访发现外周血sur-vivin表达阳性患者的复发及转移率(47.3%)明显高于阴性患者(16.6%)。提示survivin mRNA在外周血的表达与肿瘤分化、浸润深度、淋巴结转移和临床分期有关,但与肿瘤部位无明显关系。结论外周血survivin阳性患者易于复发或转移;外周血survivin的表达可作为评价结肠癌生物学行为及预后的参考指标。Objective To investigate the expression of survivin in the peripheral blood of the patients with colon cancer and its relations with recurrence and metastasis of colon cancer. Methods The expression of survivin mRNA in peripheral blood of patients with colon cancer was detected by combined use of reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay（RT-PCR-ELISA）,and the relations between its expression and the clinicopathological features were analyzed.Fifty-one patients were followed up for 36 months to analyze the relevance of the time to metastasis and relapse with the expression of survivin mRNA. Results The expression rate of survivin mRNA in the peripheral blood of the 95 patients with colon cancer was 36.8%（35 cases）.The expression rate of survivin mRNA for well to moderately differentiated cancer and poorly differentiated to undifferentiated cancer was 31.0% and 45.0%,respectively.The expression rate of the patients with tumor penetrating the serosa was 44.0%,which was higher than that of the patients with only the involvement of submucosa and muscularis（20.0%）（P0.05）.The expression rate of the patients with lymph node metastases was 47.5%,which was higher than that of the patients without lymph node metastasis（19.4%）（P0.05）.The follow-up study showed that the incidence of metastasis and relapse of the patients with survivin positive expression（47.3%） was higher than that of the patients with survivin negative expression（16.6%）.The results demonstrated that expression of surviving mRNA in the peripheral blood was associated with the degree of tumor differentiation,depth of infiltration,lymph node metastasis and clinical stages,but was irrelevant to the tumor site. Conclusions The patients with surviving positive expression in the peripheral blood are prone to metastasis and relapse.The expression of survivin in the peripheral blood may be an indicator for evaluating the biological behaviors and prognosis of colon cancer.

关 键 词：结肠肿瘤/病理学 survivin/血液 反转录-聚合酶链式反应 酶联免疫测定 

分 类 号：R735.3[医药卫生—肿瘤]