Bmi-1在胃癌中的表达及其意义  

Expression of Bmi-1 in gastric cancer and its significance

作　　者：杨国保 王洪华 张志强[2] 陆飞[2] 戴存才[3] 

机构地区：[1]江苏省响水县人民医院普通外科,江苏响水224600 [2]南京医科大学附属无锡人民医院普通外科,江苏无锡214002 [3]南京医科大学第一附属医院普通外科,江苏南京210029

出　　处：《中国普通外科杂志》2011年第10期1080-1083,共4页China Journal of General Surgery

摘　　要：目的探讨Bmi-1在胃癌组织中的表达及其与临床病理因素之间的关系。方法采用RT-PCR和Western blot技术检测44例胃癌组织和癌旁正常组织中Bmi-1的表达。结果胃癌组织Bmi-1mRNA阳性表达率为72.73%,显著高于癌旁正常组织(13.64%)(P<0.05);Bmi-1蛋白在胃癌组织中表达显著高于癌旁正常组织(P<0.05);在有淋巴结转移以及远处转移的患者中Bmi-1的表达显著高于无淋巴结转移和无远处转移阴性患者(P<0.05)。但Bmi-1的表达与患者的性别、年龄、肿瘤大小及分化程度无关,该4因素的分组间差异无统计学意义(P>0.05)。结论 Bmi-1的表达上升可能与胃癌的发生及浸润、转移有关。Objective To investigate the expression of Bmi-1（B-cell specific moloney leukemia virus insertion site 1） in gastric cancer tissues and its relation with the clinicopathological factors of gastric cancer. Methods RT-PCR and Western blot analysis were performed to detect the expression of Bmi-1 in 44 cases of gastric cancer tissues and their adjacent normal tissues. Results The positive rate of Bmi-1 mRNA expression in gastric cancer tissues was 72.73%,which was significantly higher than that in the adjacent normal tissues（14.3%）（P0.05）,and the expression of Bmi-1 protein in gastric cancer tissues was also significantly higher than that in adjacent normal tissues（P0.05）.The expression of Bmi-1 in patients with lymph node and distant metastases was significantly higher than that in patients without lymph node and distant metastases（P0.05）.The expression of Bmi-1 was irrelevant to the sex,age,tumor size or degree of tumor differentiation of the patients,because no significant differences between the groups divided according to these four factors were obtained（P0.05）. Conclusions The elevated expression of Bmi-1 may possibly be involved in the occurrence,invasion and metastasis of gastric cancer.

关 键 词：胃肿瘤 BMI-1 RT-PCR WESTERN BLOT 

分 类 号：R735.2[医药卫生—肿瘤]