大黄素对人红白血病细胞株HEL作用的研究  

Effects of Emodin on Human Erythroleukemia Cell Line HEL

作　　者：何旭春 胡建达[2] 

机构地区：[1]福建省卫生职业技术学院,福建福州350101 [2]福建医科大学附属协和医院福建省血液病研究所,福建福州350001

出　　处：《中国实验血液学杂志》2011年第5期1121-1124,共4页Journal of Experimental Hematology

摘　　要：本研究探讨大黄素(emodin)对人红白血病细胞株HEL增殖的抑制作用及其诱导凋亡的机制。应用MTT比色法检测大黄素对细胞增殖的抑制作用,AO/EB荧光染色法观察大黄素作用后细胞形态的变化,流式细胞术检测大黄素对细胞周期和凋亡的影响,Western blot法检测凋亡相关蛋白表达的变化。结果显示,大黄素能有效抑制HEL细胞的增殖,且呈浓度依赖性(r=0.995),作用48小时的IC50约为4.19μmol/L。AO/EB荧光染色法发现,大黄素作用后24小时HEL细胞发生凋亡,胞核呈致密浓染或碎片并发出黄绿色荧光;流式细胞术检测显示大黄素作用后24和48小时细胞的凋亡率分别为(27.35±1.68)%和(58.49±1.55)%,与空白对照组相比,大黄素作用后G0/G1期细胞比例增多,S期细胞比例减少(p<0.01);Akt蛋白的表达未见明显变化(p>0.05),P-Akt、P-GSK3β和HSP70蛋白的表达下调(p<0.05)。结论:大黄素能显著抑制HEL细胞的增殖,并诱导其凋亡,其机制可能与P-Akt、P-GSK3β和HSP70蛋白的表达下调有关。This study was aimed to investigate the inhibitory effect of emodin on the proliferation of HEL cells,the inducing apoptosis effect of HEL cells and their mechanisms.The proliferation inhibition was detected by MTT method;the change of morphology was observed by AO/EB stains;the cell cycle and apoptosis was analyzed by flow cytometry;the expressions of Akt,P-Akt,P-GSK3β and HSP70 proteins were determined by Western blot.The results indicated that emodin displayed significant anti-proliferative effect on HEL cells in a dose dependent manner（r=0.99）,with IC50 value of 4.19 μmol/L;AO/EB stains showed that the morphology of HEL cells obviously changed after emodin treatment for 24 hours,and at 24 and 48 hours the apoptosis rates of HEL cells treated by emodin were（27.35±1.68）% and（58.49±1.55）% respectively.Compared with blank control group,the cell ratio in G0/G1 phase increased while that in S phase decreased（p0.01）;the expression of Akt protein was not changed（p0.05）,and that of P-Akt,P-GSK3β and HSP70 proteins were down-regulated（p0.05）.It is concluded that emodin efficiently inhibits the HEL cell proliferation and induces apoptosis of HEL cells,which may be related to the down-regulation of P-Akt,P-GSK3β and HSP70 proteins expression.

关 键 词：大黄素 红白血病 HEL细胞 细胞增殖 细胞凋亡 

分 类 号：R733.73[医药卫生—肿瘤] R979.1[医药卫生—临床医学]