实时定量逆转录PCR检测慢性淋巴细胞白血病MDM4基因mRNA的表达及其临床意义  

MDM4 mRNA Levels Detected by Real-Time Quantitative RT-PCR in Chronic Lymphocytic Leukemia and Their Clinical Srgnificance

作　　者：刘玲[1] 方成[1] 董华洁[1] 王冬梅[1] 朱丹霞[1] 乔纯[1] 范磊[1] 缪扣荣[1] 刘澎[1] 徐卫[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院江苏省人民医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2011年第5期1145-1149,共5页Journal of Experimental Hematology

基　　金：国家自然基金项目(编号30871104;30971295;30971296);江苏省医学重点人才基金项目(编号RC2007042);江苏省自然科学基金项目(编号BK2010584);江苏省"青蓝工程"中青年学术带头人培养对象项目;江苏省"六大人才高峰"资助项目;南京医科大学第一附属医院创新团队工程资助项目;江苏高校优势学科建设工程资助项目

摘　　要：本研究旨在探讨慢性淋巴细胞白血病(CLL)患者鼠双微体4(MDM4)基因mRNA的表达情况及其在CLL预后中的意义。采用SYBR Green I荧光染料行实时定量逆转录PCR(qRT-PCR)方法检测66例CLL患者白血病细胞MDM4 mRNA的表达,以β-actin为内参照物,以2(-△Ct)方法计算MDM4的相对定量值,组间分析采用秩和检验。结果显示,66例CLL患者MDM4 mRNA相对表达水平的中位数为0.037098(0.088245-0.014875);具有P53基因缺失的CLL患者,其MDM4的表达明显高于无P53基因缺失的患者(0.13167 vs 0.030927)(p<0.001),具有P53基因突变的CLL患者MDM4 mRNA表达也明显高于无P53基因突变的患者(0.13167 vs0.03077)(p<0.001);此外,MDM4 mRNA的表达水平还与Binet分期(p=0.044)和ATM基因缺失(p=0.046)显著相关,但与血清乳酸脱氢酶(LDH)(p=0.216)、β2-微球蛋白(β2-MG)(p=0.314)、胸苷激酶(TK)1(p=0.300)、ZAP-70(p=0.559)、CD38(p=0.513)和免疫球蛋白重链可变区(IgVH)基因突变状态(p=0.333)无显著相关性。结论:MDM4的表达与P53基因异常关系密切,与Binet分期和ATM基因缺失相关,MDM4可能在CLL的预后中具有重要价值。This study was aimed to investigate the expression level of murine double minute 4（MDM4） mRNA in chronic lymphocytic leukemia（CLL） and its prognostic value in CLL.By means of β-actin as internal reference,the real-time quantitative RT-PCR was set up.The expression of MDM4 mRNA in 66 CLL patients was measured by fluorescence dye SYBR Green I.The dispersion of MDM4 expression ratio of groups with different prognostic factors was described by using Mann-Whitney U test.The results showed that the median MDM4 mRNA expression level was 0.037098（0.088245-0.014875） in CLL patients.The expression level of MDM4 mRNA was significantly higher in patients with P53 gene deletion than that in patients without P53 gene deletion（0.13167 vs 0.030927）（p0.001）,and also significantly higher in patients with P53 mutation than that in patients without P53 mutation（0.13167 vs 0.03077）（p0.001）.MDM4 expression was also associated with Binet stages（p=0.044） and ATM gene deletion（p=0.046）,but was not associated with LDH（p=0.216）,β2-MG（p=0.314）,TK1（p=0.300）,ZAP-70（p=0.559）,CD38（p=0.513） and IgVH mutation status（p=0.333）.It is concluded that the expression level of MDM4 is significantly higher in patients with P53 deletion or mutation.MDM4 expression is significantly associated with Binet stages and ATM gene deletion.MDM4 may be an important prognostic factor in CLL.

关 键 词：慢性淋巴细胞白血病 MDM4基因 实时定量RT-PCR 

分 类 号：R733.72[医药卫生—肿瘤]