采用小动物活体荧光成像对胃癌皮下和原位移植肿瘤生长的动态观察  

Dynamic Observation On Growth of Subcutaneous and Orthotopic Gastric Tumor by in vivo Fluorescence Imager

作　　者：彭秀华[1] 沈艳[1] 徐春华[1] 杨玉琴[1] 周文江[1,2] 

机构地区：[1]上海市公共卫生临床中心,上海201508 [2]复旦大学药学院,上海201203

出　　处：《实验动物与比较医学》2011年第5期371-375,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的 采用小动物活体荧光成像系统对裸小鼠皮下和原位移植MKN-45-Luc胃癌细胞组织瘤块后,进行肿瘤生长的动态观察.方法 将转染Luc的MKN-45胃癌细胞接种于裸小鼠皮下,成瘤后取瘤块分别接种于裸小鼠的皮下和胃部,并用活体荧光成像系统定期监测MKN-45-Luc细胞在小鼠体内成瘤的动态变化过程.结果 从第1～5周,随着肿瘤移植时间的增加,皮下和原位瘤所表达荧光素酶的面积逐渐增大,荧光光子数也逐渐增加,移植瘤体积、荧光面积与荧光光子数成正相关（r=0.882,P＜0.001）.5～6周时移植瘤体积、荧光面积与荧光光子数无相关性.结论 采用活体荧光成像系统能非常完整地观察活体动物体内胃癌的生长及转移过程.为研究MKN-45胃癌生长的生物学特性提供依据.Objective To observe the growth profile of MKN-45-Luc gastric tumor in nude mice by fluorescence imaging system of small animals in vivo, after the tumor transplantation into the nude mice through subcutaneous or orthotopic transplantation. Methods To inoculate subcutaneously the MKN- 45 gastric cancer cells which were transfected Luc into nude mice, when the tumor formed, it were harvested and inoculated subcutaneously or directly transplanted into the stomach of nude mice. AT the same time, we used the fluorescence imaging system of small animals in vivo to monitor the dynamic changes of MKN-45-Luc cells in mice. Results From the first week to the fifth week, both the luciferase expressed area of the subcutaneous and orthotopic tumor and the fluorescent intensity were gradually increased with the time increase of tumor transplantation. A positive correlation existed among the tumor volume, fluorescence area and fluorescent intensity （r=0.882, P〈0.001）, which disappeared after 5-6 weeks following the inoculation. Conclusions The fluorescence imaging systems of small animals in vivo were untilized to fully observe the growth and metastasis profile of gastric cancer, which provided the basis for investigating the biological characteristics of the MKN-45 gastric cancer.

关 键 词：活体荧光成像 皮下和原位移植 胃癌 荧光素酶 

分 类 号：R735.2[医药卫生—肿瘤]