MB-WCX联合SELDI-TOF-MS在胃癌诊断中的应用  

MB-WCX coupled with SELDI-TOF-MS:application to the diagnosis of gastric cancer

作　　者：刘博学[1] 程欢欢[2] 董召刚[3] 王华阳[2] 尹作花[3] 宋庆旭[2] 

机构地区：[1]山东大学齐鲁医院医学工程科,济南250012 [2]山东大学齐鲁医院临床基础研究所,济南250012 [3]山东大学齐鲁医院检验科,济南250012

出　　处：《山东大学学报（医学版）》2011年第9期102-105,121,共5页Journal of Shandong University:Health Sciences

基　　金：山东大学研究生自主创新基金资助(yzc10133);山东大学优秀研究生科研创新基金资助(yyx10126)

摘　　要：目的研究WCX纳米磁珠联合表面增强激光解析电离化飞行时间质谱(surface-enhanced laser desorption i-onization time-of-flight mass spectrometry,SELDI-TOF-MS)对胃癌及其癌前病变诊断的意义。方法应用纳米磁珠联合SELDI-TOF-MS对26例胃癌、35例癌前病变患者(萎缩性胃炎15例,胃溃疡20例)及30例正常人血清标本进行分析,Swiss蛋白数据库搜索鉴定差异蛋白,建立诊断模型。结果与健康对照相比较,质荷比为2 741.2、7 963.6和15 904.3的蛋白质分子随病变程度表达上调,质荷比为13 732.6的蛋白质在癌前病变组无统计学差异,但在胃癌患者中下调。质荷比2 741.2、7 963.6、15 904.3及13 732.6的蛋白质组成的诊断模型可以准确鉴别胃癌与正常对照,敏感性为92.3%,特异性为96.7%。结论 WCX纳米磁珠联合SELDI-TOF-MS是筛选胃癌早期诊断标志物的有效手段,2 741.2、7 963.6、15 904.3及13 732.6的蛋白质组成的诊断模型有可能成为潜在的胃癌早期诊断标志物。Objective To explore the validity of MB-WCX coupled with SELDI-TOF-MS on the diagnosis of gastric cancer and its precancerosis.Methods MB-WCX coupled with SELDI-TOF-MS was used to analyze the proteomic profiles of sera from 26 patients with gastric cancer,20 patients with gastric ulcer,15 patients with atrophic gastritis,and 30 healthy individuals.The differentially expressed proteins were examined by comparing to the Swiss protein database,according to their molecular weights and isoelectric points.Results Three proteins at 2 741.2,7 963.6 and 15 904.3 m/z were up-regulated in patients with gastric cancer or precancerosis,relative to healthy individuals.A fourth one at 13 732.6 m/z was down-regulated in patients with gastric cancer,but showed little difference between precancerosis and healthy conditions.These four proteins,in combination,can be used to detect gastric cancer,with a sensitivity of 92.3%,and a specificity of 96.7%.Conclusion MB-WCX coupled with SELDI-TOF-MS is an effective means to screen for biomarkers.The four low molecular weight serum proteins can be hopeful biomarkers for gastric cancer.

关 键 词：表面增强激光解析电离化飞行时间质谱 纳米磁珠 胃肿瘤 癌前状态 诊断 

分 类 号：R735.2[医药卫生—肿瘤]