转录因子Snail在乳腺癌中的表达及意义  

Expression and Significance of Transcription Factor Snail in Breast Cancer

作　　者：赵典朋[1] 申安[1] 李世平[1] 刘桂玲[1] 尹崇高[2] 

机构地区：[1]潍坊医学院附属医院肝胆外科,山东省潍坊市261031 [2]潍坊医学院护理学院外科教研室

出　　处：《中华全科医学》2011年第11期1684-1685,共2页Chinese Journal of General Practice

摘　　要：目的检测转录因子Snail mRNA及蛋白在乳腺癌中的表达,探讨Snail在乳腺癌发生发展中的作用。方法采用免疫组织化学、RT-PCR的方法检测乳腺癌、癌旁相对正常乳腺组织(>5 cm)中转录因子Snail mRNA及蛋白的表达,并分析其与临床病理参数之间的关系。结果乳腺癌组织中Snail mRNA的阳性表达率高于癌旁相对正常乳腺组织(P<0.05);乳腺癌组织中Snail mRNA的阳性表达量高于癌旁相对正常乳腺组织(P<0.05)。乳腺癌组织中Snail蛋白的阳性表达率高于癌旁相对正常乳腺组织(P<0.05),且癌组织中Snail蛋白阳性表达率与淋巴结转移有关(P<0.05),而与TNM分期、分化程度无关。结论乳腺癌的癌变、侵袭、转移可能与Snail蛋白、mRNA的高表达有关。Objective To detect the expression of transcription factor Snail mRNA in breast cancer tissues and its role in the development of breast cancer.Methods The expression of Snail protein and mRNA in breast cancer and normal breast tissue were detected by immunohistochemistry and RT-PCR.The relatives of Snail and clinicothological parameters were analyzed.Results The positive rate of Snail mRNA were significantly higher in cancer tissues than that in normal breast tissues（P0.05）;The expression of Snail mRNA were significantly higher in cancer tissues than that in normal breast tissues（P0.05）.The positive rate of Snail protein were significantly higher in cancer tissues than that in normal breast tissues（P0.05）.The expression of Snail in cancer tissue were correlated with lymphnode metastasis（P0.05） and were not correlated with TNM stage and differentiation grade.Conclusion The carcinogenesis,invasion and metastasis of breast cancer may be related to overexpression of Snail protein and mRNA.

关 键 词：SNAIL 乳腺肿瘤 MRNA 蛋白 

分 类 号：R737.9[医药卫生—肿瘤] R341[医药卫生—临床医学]