表面解吸常压化学电离质谱法直接测定宠物食品中三聚氰胺  

Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry for Direct Detection Melamine in Pet Foods

作　　者：张燕[1] 沈燕[2] 韩超 陈焕文[1] 张兴磊[1] 

机构地区：[1]东华理工大学/江西省质谱科学与仪器重点实验室,江西南昌330013 [2]温州大学化学与材料工程学院,浙江温州325035 [3]温州出入境检验检疫局,浙江温州325027

出　　处：《现代科学仪器》2011年第5期86-90,共5页Modern Scientific Instruments

基　　金：温州市科技局科研(G20090150);江西省自然科学基金(2010GZH0002)项目资助

摘　　要：采用表面解吸常压化学电离(SDAPCI)质谱法,在无需复杂样品预处理的情况下,建立直接、快速的宠物食品中三聚氰胺测定方法,并用串联质谱对测定结果进行鉴定。采用手动进样,单个样品获取信息时间少于30 s,对狗咬胶样品中三聚氰胺的回收率为89.2%～113.1%;相对标准偏差(RSD)在7.4%～9.5%之间;检出限为3.05μg/kg。应用本方法对A、B、C、D、E、F六种狗咬胶样品进行测定,测得三聚氰胺含量分别为25.4、17.3、148.4、130.6、10493.9、1168.0μg/kg,六种狗咬胶样品中三聚氰胺信号强度的相对标准偏差在4.7%～12.6%之间。本方法无需复杂的样品预处理,分析速度快,灵敏度高,在检测复杂基体样品时,特异性强,适用于批量样品的快速检测。A novel method was developed for direct and rapid detection of trace amounts of melamine in pet foods based on surface desorption atmospheric pressure chemical ionization mass spectrometry（SDAPC I-MS） without complicated sample pretreatment.Taking the advantages of multiple-stage tandem MS capability of an ion trap mass analyzer,all the m/z signals detected from dog chewing foods were simultaneously identified using the characteristic fragments of melamine,which result in confident validation of the analytical results.The average time for a single sample analysis was less than 30 seconds;the speed was largely determined by the sample loading which took about 28 s for each manual delivery.The recoveries were 89.2%~113.1%,and the relative standard deviations were 7.4%~9.5%.The limit of detection of melamine in pet foods was 3.05 μg/kg.The concentrations of melamine in six dog chewing foods,which are labelled A,B,C,D,E and F,are 25.4,17.3,148.4,130.6,10493.9,1168.0μg/kg by the developed method.The relative standard deviations of melamine＇s signal intensity were 4.7 %~12.6%.This method has the advantages of without complicated sample pretreatment,fast analysis speed,high sensitivity and specificity.Taking together SDAPCI-MSn is a powerful tool for rapid,semi-quantitative detection method for melamine analysis in pet foods.

关 键 词：表面解吸常压化学电离源 质谱 三聚氰胺 宠物食品 快速检测 

分 类 号：O657.63[理学—分析化学]