Punica granatum果实皮甲醇提取物化学成分的分离和标准化(英文)  

Isolation and Standardization of Various Phytochemical Constituents from Methanolic Extracts of Fruit Rinds of Punica granatum

作　　者：Vishal Jain Murugananthan G Deepak M GL Viswanatha D Manohar 

机构地区：[1]Department of Pharmacognosy,PES College of Pharmacy [2]Department of Phytochemistry,Natural Remedies Pvt Ltd. [3]Department of Pharmacology,PES College of Pharmacy

出　　处：《中国天然药物》2011年第6期414-420,共7页

摘　　要：AIM:The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum.METHODS:Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor;the percentage yield of the methanolic extract (MEPG) was found to be 26%;the methanolic extract was partitioned using n-butanol,ethyl acetate and water;the percentage yield of the fractions were found to be 17.16%,26.88% and 47.72% respectively.HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography.The isolated markers were characterized using UV,IR,Mass and NMR spectroscopy.RESULTS & CONCLUSION:Totally four compounds were isolated and identified as punicalin,punicalagin,gallic acid and ellagic acid based on UV,IR,Mass and NMR data,they were standardized by HPLC.AIM：The present study was undertaken to isolate and standardize the various active phytochemical constituents present in the fruit rinds of Punica granatum.METHODS：Fruit rinds of Punica granatum were dried and extracted with methanol in a static extractor;the percentage yield of the methanolic extract （MEPG） was found to be 26%;the methanolic extract was partitioned using n-butanol,ethyl acetate and water;the percentage yield of the fractions were found to be 17.16%,26.88% and 47.72% respectively.HPLC was carried out for all the fractions and the markers corresponding to the major peaks in HPLC were isolated using column chromatography.The isolated markers were characterized using UV,IR,Mass and NMR spectroscopy.RESULTS CONCLUSION：Totally four compounds were isolated and identified as punicalin,punicalagin,gallic acid and ellagic acid based on UV,IR,Mass and NMR data,they were standardized by HPLC.

关 键 词：Punica granatum Punicalin PUNICALAGIN Gallic acid and ellagic acid 

分 类 号：R284[医药卫生—中药学]