急性淋巴细胞白血病患者CD4＋CD25＋调节性T细胞水平变化  

CD4＋CD25＋ regulatory T cells＇level in peripheral blood of patients with acute lymphocytic leukemia

作　　者：巫翠萍[1] 覃西[1] 巫翠云[4] 何金龙[1] 周海燕[2] 温晓梅[3] 

机构地区：[1]海南医学院附属医院检验科,海口570102 [2]海南医学院附属医院血液内科,海口570102 [3]海南医学院附属医院儿科,海口570102 [4]海口市人民医院检验科,570208

出　　处：《国际免疫学杂志》2011年第6期390-392,共3页International Journal of Immunology

基　　金：海南省卫生厅科研立项课题（琼卫2008-55）

摘　　要：目的观察急性淋巴细胞白血病（ALL）患者外周血中CD4＋CD25＋调节性T细胞（Treg）的变化，探讨其临床意义。方法收集47例ALL初诊患者组、13例经化疗完全缓解组、9例未缓解组及20例健康对照组抗凝血，采用流式细胞仪检测CD4＋CD25＋Treg的水平。结果CD4＋CD25＋Treg比例在ALL初诊组、治疗后完全缓解组、治疗后未缓解组和健康对照组分别为（5．78±2．42）％、（4．39±1．75）％、（6．76±2．57）％和（3．90±0．79）％。初诊组和未缓解组明显高于健康对照组（P=0．001，P=0．000），而完全缓解组与健康对照组比较无统计学意义（P=0．130）；化疗后完全缓解组和未缓解组与初诊组均无统计学意义（P=0．058，P=0．278），而完全缓解组明显低于未缓解组（P=0．018）。结论ALL患者外周血中高表达CD4＋CD25＋Treg可能是ALL免疫抑制的一个重要原因，其变化对于ALL的疗效评价及预后判断有一定的意义。Objective To observe CD4＋CD25＋ regulatory T cells＇level in peripheral blood of patients with acute lymphocytic leukemia and explore its clinical value. Methods Coagulated blood was collected from 4 groups people, including 47 ALL patients before chemotherapy, 13 ALL patients with complete remission after chemotherapy, 9 ALL patients without remission and 20 cases of healthy control. CD4＋CD25＋ regulatory T cells in peripheral blood were detected using flow cytometry. Results CD4＋CD25＋ regulatory T cells＇levbl in peripheral blood of ALL patients before chemotherapy, complete remission, without remission after chemotherapy and healthy control was （5.78± 2.42 ） %, （4.39 ± 1.75 ） %, （ 6.76± 2.57 ） % 和 （ 3.90 ± 0.79 ） % respectively. CD4＋CD25＋ regulatory T cells＇level of ALL patients before chemotherapy and without remission after ehemotherapy was higher than that of healthy control（ P = 0. 001, P = 0. 000 ）. There was no signifieant differenee between ALL patients with complete remission after chemotherapy and healthy control（P = 0.130）. Before and After chemotherapy, Whether remission or not, CD4＋CD25＋ regulatory T cells＇level had not the obvious difference（P =0. 058 ,P = 0. 278 ）. But patients with complete remission was lower than whom without remis- sion（p = 0. 018）. Conclusions CD4＋CD25＋ regulatory T cells High-expression in peripheral blood of ALL patients is probably important in ALL immunological suppression. To a certain extent, it is valuable to assess healing effect and decide prognosis of ALL.

关 键 词：急性淋巴细胞白血病 CD4+CD25+T调节细胞 流式细胞仪 

分 类 号：R733.71[医药卫生—肿瘤]