PSIIP680 ChlaAP基因在水稻叶绿体基因组中的定位  

Location of the PSIIP680 ChlaAP Gene in the Chloroplast Genome of Rice

作　　者：朱嘉晖 李继耕 孔繁瑞[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《Acta Genetica Sinica》1990年第5期377-382,共6页

摘　　要：本实验总结出一套水稻叶绿体DNA的提取方法,并获得清晰的叶绿体DNA限制性内切酶图谱。Southern杂交结果表明,菠菜PSIIP680ChlaAP基因探针与水稻叶绿体DNA的Pst-1,Pst-14,Pvu-2和Sal-1片段的部分顺序有较高的同源性。根据Hirai和赵衍的水稻叶绿体基因组物理图,可以确定该基因位于紧靠RuBPCaseLS基因,距反向重复区约26kb处。高等植物叶绿体基因组中这种基因排列方式还未见报道。A simple method for the preparation of chloroplast DNA (ctDNA) from rice has been developed. The rice ctDNA prepared with this method has successfully been used in restriction enzyme mapping anl Southern transfer. The gene probe of PSIlP680ChlaAP from spinach was hybridized with Pst-1, Pst-14, Pvu-2 and Sal-1 fragments of rice ctDNA in Southern hybridization. According to the physical map of rice ctDNA drawn by Hirai and Zhao Yan, this gene located at the site nearby the RuBPCase LS gene and about 26 kb from the nearer one of the inverted repeat region in the chloroplast genome of rice. The arrangement pattern oi these genes was observed in chloroplast genome in higher plants for the first time.

关 键 词：水稻 叶绿体基因组 基因定位 

分 类 号：S511.103.2[农业科学—作物学]