鲫鱼组织中辛硫磷残留量测定的基质固相分散-高效液相色谱法  

Determination of phoxim in tissues of crucian carp(Carassius auratus) by matrix solid-phase dispersion and high performance liquid chromatography

作　　者：石旺荣[1] 邱朝坤[2] 刘荣飞[1] 肖梦玲[1] 郭静[1] 刘晓宇[1] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]华中农业大学楚天学院食品与生物科技学院,武汉430205

出　　处：《食品科技》2011年第11期297-300,共4页Food Science and Technology

基　　金：湖北省自然科学基金项目(2006ABA165;2009CDB410)

摘　　要：建立了一种测定鲫鱼组织中(肝脏、肌肉、脑和鳃)辛硫磷残留量的基质固相分散-高效液相色谱方法,以1.5 g弗罗里硅土为吸附剂,以8 mL二氯甲烷为洗脱剂进行洗脱,液相色谱检测。色谱条件为:Agilent C18色谱柱,甲醇+水(75+25)为流动相,流速为1.2 mL/min,柱温设置在35℃,检测波长为280 nm。各个组织的回收率在0.525 mg/kg和2.1 mg/kg的加标水平下均在80%～110%之间,相对标准偏差<15%,检测限为0.02 mg/kg。方法精确可靠,可用于鲫鱼组织中辛硫磷残留量的检测。A simple method based on matrix solid-phase dispersion（MSPD） and high performance liquid chromatography （HPLC） for quantification of phoxim in tissues（liver, muscle, brain and gill） of crucian carp （Carassius auratus） is described. The operational MSPD conditions were 1.5 g of Florisil as dispersant and subsequent extraction with 8 mL of dichloromethane. Chromatographic separation was obtained using an Agilent C18 column. The mobile phase consisted of an isocratic mixture of methanol ＋ water（75＋25）. The flow rate was kept at 1.2 mL/min and the column temperature was maintained at 35 ℃. Detection was conducted at 280 nm. At two spiking levels （0.525 mg/kg and 2.1 mg/kg）, the recoveries were in the range of 80%-110% with relative standard deviations 〈 15% for all tissues. The limits of detection were 0.02 mg/ kg for all tissues. The method was reliable for routine analysis of phoxim in the body of fish.

关 键 词：辛硫磷 测定 鲫鱼 基质固相分散(MSPD) 高效液相色谱(HPLC) 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]