NKX2.5基因修饰骨髓间充质干细胞对心肌梗死大鼠心功能的影响  

Heart function effect of mesenchymal stem cells modified by NKX2.5 in infracted myocardium in rats

作　　者：赵东海[1] 张磊[2] 李大鹏[3] 

机构地区：[1]吉林医药学院病理学教研室,吉林吉林132013 [2]吉林医药学院生化检验教研室,吉林吉林132013 [3]吉林医药学院附属医院呼吸内科,吉林吉林132013

出　　处：《中国医药导报》2011年第32期18-20,F0003,共4页China Medical Herald

基　　金：吉林省教育厅"十一五"科学技术研究项目资助课题(2009309);吉林市科技发展计划项目资助课题(20090602)

摘　　要：目的:以NKX2.5融合绿色荧光蛋白基因(NKX2.5-pEGFP)质粒转染大鼠骨髓间充质干细胞(MSCs),将基因修饰后MSCs移植入大鼠心肌梗死模型中,评价其对心功能的影响。方法:体外分离培养大鼠MSCs,构建NKX2.5-pEGFP基因质粒,以lipo2000将质粒转染入MSCs。将基因修饰后MSCs植入心肌梗死后10 d的大鼠心肌梗死区(MSCs组,n=20),同时将注射等体积无血清改良依格尔(DMEM)培养液于心肌梗死区的大鼠作为DMEM组(n=20)。移植注射后4周,通过检测左心室射血分数(LEVF)、左室缩短分数(FS)等研究其对心功能的影响。结果:移植4周后,MSCs组大鼠LEVF[(79±4)%]较DMEM组[(47±3)%]显著提高(P<0.01),MSCs组大鼠FS[(48±9)%]较DMEM组[(25±6)%]显著提高(P<0.01)。结论:移植NKX2.5基因修饰后的MSCs能够改善心肌梗死大鼠心功能。Objective： To transfect NKX2.5-pEGFP plasmid into mesenchymal stem cells （MSCs） and transplant the gene modified MSCs in rat myocardial infarction model to estimate the effect on heart function. Methods： MSCs were isolated cul- ture in vitro. NKX2.5-pEGFP plasmid was constructed and transfected into MSCs. The gene modified MSCs was transplanted in the area of rat myocardial infarction model after 10 days （MSCs group, n=20）. The equal volume of none blood serum DMEM was injected in myocardial infarction model as DMEM group （n=20）. The effect on heart function was estimated by detecting left ventricular ejection fraction （LVEF） and fractional shortening （FS） after injecting 4 weeks. Results： 4 weeks after transplantation, the LEVF value of MSCs group [（79±4）%] increased significantly compared to that of DMEM group [（47±3）%] （P〈0.01） and the FS value of MSCs group [（48±9）%] increased significantly compared to that of DMEM group [（25±6）%] （P〈0.01）. Conclusion： The myocardial infarction rat heart function can be improved by transplanting NKX2.5 gene modified MSCs.

关 键 词：NKX2.5基因 骨髓间充质干细胞 心肌梗死 心功能 

分 类 号：R541[医药卫生—心血管疾病]