FISH在产前羊水细胞染色体数目异常诊断中的应用观察  

Application of FISH in prenatal diagnosis of chromosome number abnormality in amniotic fluid cells

作　　者：张艳丽 李华锋 高刚 

机构地区：[1]临沂市妇幼保健院,山东临沂276000

出　　处：《山东医药》2011年第38期20-21,共2页Shandong Medical Journal

基　　金：卫生部科研基金资助项目(WKJ2007-3-001)

摘　　要：目的观察应用荧光原位杂交(FISH)技术产前诊断羊水细胞染色体数目异常的效果。方法唐氏综合征筛查高危或高龄(≥35岁)孕妇1 121例,经腹部穿刺抽取羊水,应用FISH技术进行羊水细胞染色体数目检测,并将其结果与羊水细胞常规G显带核型分析结果作比较。结果均获得诊断结果,发现16例异常胎儿,其中7例为21三体,4例为18三体,5例为其他异常。FISH检测与核型分析结果一致。结论用FISH产前诊断羊水细胞染色体数目异常效果满意。Objective To observe effect of fluorescence in situ hybridization（FISH） on prenatal diagnosis of abnormal number of chromosomes in amniotic fluid cells.Methods The amniotic fluid of 1 121 cases of pregnant women with down syndrome screening in high-risk or age higher than 35 years old,were got by amniocentesis,and udenvent rapid prenatal diagnosis by FISH.Then the G banding karyotypes from standard cytogenetic analysis after cultured amniotic fluid cells were compared to the FISH results.Results 16 cases were found abnormal result,including 7 cases of trisomy 21,4 cases of trisomy 21,and other 5 cases with abnormal.It was consistent with G banding karyotypes results.Conclusion Prenatal diagnosis of chromosome humber sbnormality by FISH is satisfactory.

关 键 词：荧光原位杂交技术 产前诊断 染色体 羊水细胞 

分 类 号：R71[医药卫生—妇产科学]