OPN在喉癌组织中的表达变化及LV-shOPN对喉癌Hep-2细胞侵袭转移的影响  

Expression of osteopontin in laryngeal carcinoma tissues and effect of LV-shOPN on infiltration and metastasis of laryngeal carcinoma Hep-2 cells

作　　者：陈剑秋[1,2] 朱敏辉[2] 邱晓霞[2] 夏思文[2] 刘达[1] 陈世彩[2] 郑宏良[2] 

机构地区：[1]济南军区总医院,济南250031 [2]第二军医大学附属长海医院

出　　处：《山东医药》2011年第42期8-10,共3页Shandong Medical Journal

摘　　要：目的观察人喉鳞状细胞癌(LSCC)组织中骨桥蛋白(OPN)的表达变化及慢病毒shOPN对喉癌Hep-2细胞侵袭转移的影响。方法采用免疫组化SP法,检测44例LSCC及癌旁正常组织中的OPN。干扰OPN慢病毒载体(LV-shOPN)转染Hep-2细胞,Western blot检测该细胞其中的OPN,MTT法检测Hep-2细胞增殖活性,Transwell小室实验检测Hep-2细胞的侵袭力。结果 LSCC组织和癌旁正常组织中OPN阳性表达率分别为75.0%(33/44)、13.6%(6/44),两者相比,P<0.01;OPN的表达与LSCC颈部淋巴结转移、临床分期、病理组织学分级有关(P均<0.01)。LV-shOPN转染后Hep-2细胞中OPN蛋白表达下降、细胞增殖活性及侵袭力降低(P均<0.05)。结论 OPN在喉癌组织中的表达高于癌旁正常组织,可能参与了喉癌的发生、发展和转移;LV-shOPN能够有效抑制Hep-2细胞的生长和侵袭转移。Objective To observe the expression of osteopontin（OPN） in the largngeal squamous cell carcinoma（LSCC） tissues,and the effect on the proliferation and invasiveness of laryngeal cancer Hep-2 cells of lentivirus vector with interference OPN（LV-shOPN）.Methods SP immunohistochemical method was used to detect the OPN of 44 LSCC and adjacent tissues.LV-shOPN was transfected into Hep-2 cells.OPN in the Hep-2 cells was detected by Western blot was detected by Transwell assay,the proliferation of Hep-2 cells was examined by MTT.The invasion ability of Hep-2 cells was detected by Transwell assay.Results The positive rate of OPN in LSCC and adjacent tissue was 75.0 %（33/44）and 13.6%（6/44）respectively,the two rates were compared,P0.01.The OPN expression levels were significantly correlated with the presence of lymph node,clinical stage and histopathology grade of LSCC,all P0.01.After LV-shOPN transfection,the expression of OPN was reduced and ability of the proliferation and invasiveness was decreased in Hep-2 cells（all P0.05）.Conclusions OPN expression is higher in LSCC than that in adjacent tissue,and maybe participated in the development and metastasis of LSCC.LV-shOPN can inhibit efficiently the proliferation and invasiveness of Hep-2 cells.

关 键 词：骨桥蛋白 喉肿瘤 HEP-2细胞 RNA干扰 肿瘤转移 

分 类 号：R739.65[医药卫生—肿瘤]