牙龈卟啉单胞菌脂多糖对人主动脉内皮细胞表达ICAM-1影响的研究  

Porphyromonas gingivalis LPS induces ICAM-1 expression in human aortic endothelial cells

作　　者：刘斌[1] 程岚[2] 刘大力[2] 汪嘉[1] 张秀丽[3] 束蓉[2] 梁景平[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.牙体牙髓科,上海200011 [2]上海交通大学医学院附属第九人民医院.牙周病科,上海200011 [3]上海市口腔医学研究所上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2011年第10期568-572,共5页Chinese Journal of Conservative Dentistry

基　　金：上海市科委基础重点研究项目(09JC1409100)

摘　　要：目的:分别观察牙龈卟啉单胞菌(P.gingivalis)脂多糖(LPS)和大肠杆菌(E.coli)LPS对人主动脉内皮细胞(HAECs)在转录和翻译水平表达细胞间粘附分子-1(ICAM-1)的影响。方法:在体外分别以P.gingivalisLPS和E.coliLPS刺激HAECs,实时荧光定量PCR技术检测ICAM-1基因表达,蛋白质印迹技术检测ICAM-1膜蛋白表达。采用SPSS18.0软件包中ANOVA对数据进行分析。结果:P.gingivalisLPS作用6 h后开始诱导ICAM-1 mRNA表达,14 h达到高峰,22 h仍有高表达,各时间点与未刺激组相比,差异均有统计学意义(P<0.05);而E.coliLPS作用2 h后即开始诱导ICAM-1 mRNA表达,14 h达到高峰,22 h仍有高表达,各时间点与未刺激组相比差异均有统计学意义(P<0.05)。两种LPS均能在蛋白水平上调ICAM-1的表达,这种上调作用呈浓度和时间依赖,且P.gingivalisLPS的作用较之E.coliLPS要缓和。结论:P.gingivalisLPS能够在转录和翻译水平上调HAECs表达ICAM-1,提示P.gingivalisLPS在动脉粥样硬化(AS)的发生发展过程中可能发挥了重要作用。E.coliLPS可能与急性炎症性疾病有关而与AS无关。AIM： To observe the effects of Porphyromonas gingivalis（P.gingivalis）Lipopolysaccharide（LPS）and E.coli LPS on mRNA and protein expressions of intercellular adhesion molecule-1（ICAM-1）in human aortic endothelial cells（HAECs）.METHODS： HAECs were challenged by P.gingivalis LPS or E.coli LPS in vitro.ICAM-1 mRNA was measured by realtime PCR.The protein expression of ICAM-1 was detected by Western blotting assay.The data was statistically analyzed by SPSS 18.0 software package.RESULTS： P.gingivalis LPS as well as E.coli LPS enhanced both mRNA and protein expression of ICAM-1 in HAECs.The up-regulation of ICAM-1 expression induced by P.gingivalis LPS was moderate,whereas E.coli LPS induced ICAM-1 expression in a relatively acute manner.CONCLUSION： P.gingivalis LPS but not E.coli LPS plays an important role in the development and progression of atherosclerosis partly through upregulation of ICAM-1.

关 键 词：牙龈卟啉单胞菌 脂多糖 人主动脉内皮细胞 细胞间粘附分子-1 

分 类 号：R780.2[医药卫生—口腔医学]