Matrilysin在胃癌中表达及临床意义——判断侵袭、转移、预后的指标  

Expression of Matrilysin in Gastric Cancer and Its Significance:An Indicator for Determining Invasion,Metastasis,and Prognosis

作　　者：黄宝俊[1] 赵雨杰[2] 张玉魁[2] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001 [2]中国医科大学生物芯片中心,辽宁沈阳110001

出　　处：《中国普外基础与临床杂志》2011年第10期1063-1068,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(项目编号:30672050)~~

摘　　要：目的全面了解Matrilysin在胃癌组织中的表达情况,分析其与胃癌侵袭、转移和预后的相关性。方法以52例胃癌患者为研究对象,分别应用RT-PCR、Western blot及免疫组织化学方法检测Matrilysin在胃原发癌、胃正常黏膜及淋巴结转移癌组织中的表达,对所有病例进行随访,分析Matrilysin与胃癌侵袭、转移和预后的相关性。结果 Matrilysin在胃原发癌和淋巴结转移癌组织中表达明显增高,而在胃正常黏膜组织中表达较弱或缺失,差异有统计学意义(P<0.05);其mRNA和蛋白质表达水平具有较高的一致性(P<0.001)。Matrilysin表达在胃原发癌为浸润型癌、癌侵透浆膜、浆膜受侵面积>20cm2、淋巴结分期晚者(N3)中明显增高(P<0.001)。Matrilysin高表达组患者5年生存率为34.1%,而Matrilysin低表达组为55.6%,差异有统计学意义(χ2=9.778,P=0.002)。结论 Matrilysin基因表达与胃癌的侵袭、转移和预后有关,是反映胃癌恶性生物学行为较好的分子指标。Objective To study the expression of matrilysin in gastric cancer and to evaluate the correlation between its expression and invasion,metastasis,and prognosis. Methods A total of 52 patients with gastric cancer were selected and followed up.The expressions of matrilysin in gastric primary focus,normal gastric mucosa,and metastatic lymph nodes were examined by reverse transcription-polymerase chain reaction（RT-PCR）,Western blot,and immunohistochemistry,respectively.The correlations between matrilysin expression and tumor invasion,metastasis,and prognosis were assessed. Results The expressions of matrilysin in gastric primary focus and metastatic lymph nodes significantly increased,while decreased or loss in normal gastric mucosa（P0.001）.The higher concordance was seen between the levels of mRNA and protein（P0.001）.Among patients with infiltrating type,penetrated serosa,area of serosa involved more than 20 cm2,and metastatic lymph nodes more than 7,the expression of matrilysin was significantly higher（P0.01）.The survival rate of patients with matrilysin higher expression（34.1%） was significantly lower than that with matrilysin lower expression（55.6%）,χ2=9.778,P=0.002. Conclusions Up-regulated expression of matrilysin plays an important role in tumor invasion,metastasis,and poor prognosis,and it is a good molecular marker to reflect the biological behaviors of gastric cancer.

关 键 词：胃癌 Matrilysin基因 侵袭 转移 预后 

分 类 号：R735.2[医药卫生—肿瘤]