慢性胰腺炎的猫模型制作及磁共振影像表现  

Animal model of chronic pancreatitis in cats and MR imaging features

作　　者：张霆霆[1,2] 黄志军 王莉[1] 陆建平[1] 

机构地区：[1]第二军医大学长海医院医学影像科,上海200433 [2]上海市交通大学医学院附属新华医院影像科 [3]江苏省盐城市第一人民医院普外科

出　　处：《中华胰腺病杂志》2011年第5期346-348,共3页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（81070371）

摘　　要：目的制备猫慢性胰腺炎（CP）模型，观察其MRI与MRCP的影像学表现。方法32只猫按数字表法随机分为对照组及制模后3、5、7周组。采用胰管不全结扎法制备CP模型，术后3、5、7周行MRI平扫及MRCP检查，观察胰腺形态，测量胰管直径及感兴趣区（ROI）的T1信号强度值（T1s），计算同层胰腺及肝脏T1s比值。结果制模的24只猫中存活19只，其中15只形成CP，病理证实轻、中、重度cP分别为7、5、3只，制模成功率为62．5％。在MRI上，猫的正常胰腺显示清晰，T1加权像信号强度高于肝脏，12加权像信号强度低于肝脏；在MRCP图像上，4只正常猫显示主胰管，胰管最大径（0．79±0．18）mm，并可见胰管及胆总管共同开口于十二指肠降部。正常胰腺及轻、中、重度CP感兴趣区的rT1s值分别为1．03±0．06、0．95±0．08、0．90±0．10、0．80±0．11，各CP组与正常对照组间差异均有统计学意义（t=2．18，P〈0．05；t=2．89，P〈0．05；t=4．63，P〈0．01）；胰管最大径分别为（0．79±0．18）、（0．95±0．24）、（1．26±0．31）、（2．67±0．71）mm，中、重度CP组与正常对照组间差异均有统计学意义（P〈0．05或〈0．01）。结论胰管不全结扎可制备猫的CP模型。猫的胰腺解剖形态、CP的MRI及MRCP表现与人类相似。Objective To establish an animal model of cat with chronic pancreatitis （CP）, and to investigate the imaging features of MRI and MRCP of normal pancreas and CP. Methods Thirty two cats were randomly divided into control group （n = 8） and experimental group （n = 24） （3, 5, 7 weeks after model establishment, eight eats in every group）. Partial ligation of the pancreatic duet was used to induce chronic panereatitis. All eats underwent MRI plain scanning, MRCP scanning 3, 5, and 7 weeks after modeling to observe the pancreas morphology. The values of T1 signal intensity of pancreas were obtained using a region of interest （ROI） method, and the diameter of pancreatic duet was measured. The ratios of T1 signal intensity （ rT1 s） between pancreas and liver were calculated and compared. Results Nineteen of 24 eats survived, and 15 eats developed CP, and the pathologic examinations showed mild, middle, severe CP developed in 7, 5, 3 cats, suggesting a successful model induction rate of 62.5%. The pancreases of eats were displayed clearly on MRI. The signal intensity of pancreas was higher than that of liver on T1WI, whereas it was lower than that of liver on T2WI. Meanwhile, there were four eats＇ pancreatic duets were shown on MRCP, the maximum diameter of pancreatic duet was （0.79 ± 0. 18） ram, and the opening orifice of pancreatic duet was located at descendant duodenum with common bile duet. The rTls of ROI of normal pancreas, mild, middle, severe CP was 1.03±0.06,0.95 ± 0.08,0.90 ± 0.10,0.80 ± 0.11, and the difference among these groups was statistically significant （ t = 2.18, P 〈 0.05 ; t = 2.89, P 〈 0.05 ; t = 4.63, P 〈 0. 01 ）. The maximum diameter of pancreatic duct was （0.79±0.18）, （0.95±0.24）, （1.26 ±0.31）, （2.67 ±0.71）mm, and maximum diameter of pancreatic duct of middle, severe CP was significantly different when compared with that of normal pancreas （ P 〈 0.05 or 〈 0.01 ）. Conclusions The method of partial ligation of pancreatic duct ca

关 键 词：猫 胰腺 磁共振成像 胰胆管造影术 磁共振 慢性胰腺炎 

分 类 号：R-332[医药卫生] R576