乳腺癌组织多药耐药相关蛋白表达及其与化疗疗效的相关性研究  

Expression of drug resistance related proteins in breast cancer tissues and its relationship with chemotherapy therapeutic effect

作　　者：刘新兰[1] 陈萍[1] 崔洁[2] 张桂香[3] 

机构地区：[1]宁夏医科大学附属医院肿瘤内科,宁夏银川750004 [2]宁夏医科大学附属医院医学实验中心,宁夏银川750004 [3]宁夏医科大学研究生院,宁夏银川750004

出　　处：《中华肿瘤防治杂志》2011年第17期1354-1357,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：宁夏回族自治区高等学校科学技术研究项目(J2006114)

摘　　要：目的:探讨乳腺癌组织中多药耐药蛋白(P-gp)、乳腺癌耐药蛋白(BCRP)和肺耐药蛋白(LRP)的表达及其与乳腺癌化疗疗效的关系。方法:免疫组化法检测126例乳腺癌和42例癌旁组织中P-gp、BCRP和LRP蛋白的表达。结果:乳腺癌组织中P-gp、BCRP及LRP阳性表达率分别为41.26%(52/126)、38.89%(49/126)和65.87%(83/126),明显高于癌旁组织的14.28%(6/42)、16.67%(7/42)和19.05%(8/42),χ2值分别为10.147、7.020和27.820,P<0.05;5年内复发转移和无复发转移者P-gp阳性表达率差异有统计学意义(P=0.001),BCRP及LRP阳性表达率差异均无统计学意义,P>0.05。5年总生存率及无病生存率P-gp和BCRP阴性表达者明显高于阳性,χ2值分别为24.17、4.43、12.13和4.22,P值均<0.05;伴有2及3个耐药相关蛋白共表达的乳腺癌患者5年无病生存期及总生存期均较1或3个者明显缩短,χ2值分别为7.43和14.30,P值均<0.05。结论:P-gp、BCRP和LRP均参与了乳腺癌多药耐药,P-gp阳性表达可作为评价乳腺癌术后采用蒽环类化疗方案疗效判断的指标。OBJECTIVE： To investigate the expression and its relationship with chemotherapy therapeutic effect of multidrug resistance protein（P-gp）,breast cancer resistance protein（BCRP）,lung cancer resistance protein（LRP） in the breast cancer tissue.METHODS： To examine the expression level of P-gp,BCRP and LRP in 126 case breast cancer tissues and 42 case paratumor tissues by immunohistochemistry（IHC）.RESULTS： The positive expression rate of P-gp,BCRP and LRP were 41.26%（52/126）,38.89%（49/126） and 65.87%（83/126）;The positive expression rate of breast cancer tissues were much higher than paratumor tissues（14.28%（6/42）,16.67%（7/42）,19.05%（8/42））,and there were significantly differences（χ2 values were 10.147,7.020 and 27.820,and all P valuse were 0.05）.The 5-year recurrence,metastasis of P-gp negative cases was significantly lower than that of positive cases after chemotherapy（P=0.001）.The positive expression rate of BCRP and LRP were no significant difference recurrence metastasis and un recurrence metastasis after chemotherapy in 5-year（P0.05）.The carcinoma which negatively expressed P-gp and BCRP had a longer 5-year both disease-free survival rate and overall survival rate than those of positive expression（χ2 values were 24.17,4.43,12.13 and 4.22,all P values were 0.05）.The 5-year both disease-free survival and overall survival of 2 multi-protein and 3 multi-protein positive cases were significantly lower than that of a single protein expression and three negative cases（χ2 values were 7.43 and 14.30,and all P values were0.05）.CONCLUSIONS： The three drug-resistance proteins are involved in the formation of multidrug resistance of breast cancer.P-gp expression may help predict of breast cancer chemotherapy therapeutic effect.

关 键 词：乳腺肿瘤 多药耐药相关蛋白 免疫组织化学方法 

分 类 号：R737.9[医药卫生—肿瘤]