宫颈上皮内瘤样病变血清多肽谱图与其转归的相关性分析  

Relationship between serum peptide profiling and biological behavior of cervical intraepithelial neoplasia

作　　者：刘芸[1] 李艳[2] 李长东[1] 王建东[1] 段华[1] 王杰[2] 张为远[1] 

机构地区：[1]首都医科大学附属北京妇产医院妇科微创中心,北京100008 [2]军事医学科学院国家仪器检测分析中心蛋白质组学国家重点实验室,北京100039

出　　处：《中华肿瘤防治杂志》2011年第17期1373-1377,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：北京市科委重大项目(D0906008040491)

摘　　要：目的:探讨应用血清多肽谱图分析宫颈上皮内瘤样病变(CIN)的血清多肽谱图的变化,寻找预测CIN转归的方法。方法:使用基质辅助激光解吸附电离串联飞行时间质谱技术(MALDI-TOF-MS),选用磁珠检测34例宫颈鳞癌患者、31例健康女性和128例CIN患者的血清多肽学谱图,建立宫颈鳞癌的诊断模型。分析血清多肽谱图与CIN转归的关系。结果:建立宫颈鳞癌的血清多肽谱图诊断模型,盲筛验证模型敏感性92.9%(13/14),特异性100.0%(14/14),阳性预测值96.4%。CIN级别升高其血清多肽谱图进入宫颈鳞癌模型的数量增加,CIN1组(74.4%)和CIN3组(12.5%)比较差异有统计学意义,P<0.01。随访18～24个月,进入鳞癌组CIN病变加重的比例高于后者(35.7%vs 12.2%,P<0.05),病变好转的概率低于后者(7.1%vs 44.9%,P<0.01)。结论:根据血清多肽学谱图进行分类,可将CIN分为高危和低危组,预测其转归,为制定CIN患者的个体化治疗方案提供依据。OBJECTIVE： To explore the feasibility to predict the biological progression of CIN by serum peptide profiling.METHODS：Serum samples from 34 patients with cervical squamous cell cancer（SCC）,128 patients with cervical intraepithelial neoplasia（CIN） and 31 healthy controls were analyzed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.Then,a serum peptide profiling diagnosis model of SCC was established.The relationship between the serum peptide profiling and the biological progression of CIN were analyzed by follow-up.RESULTS：The serum peptide profiling diagnosis model of SCC was established,the blinded test yielded a sensitivity of 92.9%（13/14）,a specificity of 100.0%（14/14）,and an accuracy rate of 96.4%.Comparing CIN to the model,CIN3 was more likely to be divided into SCC model than CIN1（74.4% vs 12.5%,P0.01）.After 18-24 months follow-up,those CIN which was divided to SCC had higher risk to progress than those which was not（35.7% vs 12.2%,P0.05）,and lower possibility to regress（7.1% vs 44.9%,P0.01）.CONCLUSIONS： It is demonstrated that the serum peptide profiling model can predict CIN biological progress by dividing CIN to high and low risk groups,which will be helpful to modify the individual treatment for CIN patients.

关 键 词：宫颈上皮内瘤样病变 癌 鳞状细胞 宫颈肿瘤 蛋白质组学 质谱技术 

分 类 号：R737.33[医药卫生—肿瘤]