Gradient decrease in telomerase and its RNA in progressive passage of human endometrial stromal stem cells  

作　　者：Yang Xinyuan Wang Wei Wang Yueling Chen Wei Li Xu 

机构地区：[1]Department of Gynaecology and Obstetrics, First Affiliated Hospital, School of Medicine, Xi＇an Jiaotong University, Xi＇an 710061, China [2]Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Xi＇an Jiaotong University, Xi ＇an 710061, China [3]Department of Clinical Laboratory, First Affiliated Hospital, School of Medicine, Xi＇an Jiaotong University, Xi ＇an710061, China

出　　处：《Journal of Medical Colleges of PLA(China)》2011年第5期254-263,共10页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(30801238)

摘　　要：The purpose of this study was to explore the change of telomerase in passage from human endometrial stromal stem cells isolated from human endometrium.Telomerase activity of cultured endometrial stromal cells was assessed at mRNA and protein levels using RT-PCR and immunohistochemistry technique.Telomerase mRNA and protein levels were higher at early passages,and then had a gradually decreased trend of immunoreactive intensity and gradually weakened positive cells with progressive passage in endometrial stromal stem cells.These results suggest that telomerase is contributed to the insenecence of endometrial stem cell.The purpose of this study was to explore the change of telomerase in passage from human endometrial stromal stem cells isolated from human endometrium. Telomerase activity of cultured endometrial stromal cells was assessed at mRNA and protein levels using RT-PCR and immunohistochemistry technique. Telomerase mRNA and protein levels were higher at early passages, and then had a gradually decreased trend of immunoreactive intensity and gradually weakened positive cells with progressive passage in endometrial stromal stem cells. These results suggest that telomerase is contributed to the insenecence of endometrial stem cell

关 键 词：Endometrial stromal cell TELOMERASE Cell culture 

分 类 号：Q55[生物学—生物化学] Q254