新型动态载荷与循环灌流生物反应器系统的设计  

Design of a new dynamic load and circulating-perfusion bioreactor system

作　　者：陈学忠[1,2] 侍才洪[1] 李瑞欣[1] 郭勇[1] 李志宏[1] 王亮[1] 张西正[1] 

机构地区：[1]军事医学科学院卫生装备研究所,天津300161 [2]南方医科大学生物医学工程学院,广州510515

出　　处：《医用生物力学》2011年第5期441-447,共7页Journal of Medical Biomechanics

基　　金：国家自然科学基金重点资助项目(10832012);天津市自然科学基金面上项目(09JCYBJC14100)

摘　　要：目的设计一套动态应变加载系统和三维灌流培养系统,并测试其性能。方法明确动态载荷与循环灌流生物反应器的设计原则;设计并构建动态应变加载系统和三维灌流培养系统,研制供三维灌流与加载专用的培养舱;测定培养系统的无菌效果以及其应变加载的精确度和稳定性,进行组织工程骨的初步培养观察。结果该生物反应器可为组织培养物提供不同大小和频率的压应变以及不同流量的灌流条件,可控精度高、操作简单可行、性能稳定;连续运转5 d后,培养液中无细菌生长。初步培养结果表明,体外构建的组织工程骨在生物反应器中培养10 d,其成骨细胞的增殖和成骨活性明显高于静态培养和单纯灌流培养。结论该生物反应器可以成为三维条件下进行骨细胞生物力学研究的一种较理想的动态培养与应变加载装置。Objective To design and build a new dynamic load and circulating perfusion bioreactor system and test its performance.Methods The design principle of the bioreactor system was specified and the dynamic strain loading system and 3D perfusion culture system were designed and built accordingly.A special culture chamber for 3D perfusion and compressive loading was also developed.The sterility of the culture chamber and the accuracy and stability of the strain loading were measured,and the result from the culture of tissue engineering bone was preliminarily observed.Results This bioreactor system could provide compressive strains with different magnitudes and frequencies,as well as perfusions under different flow conditions.It could be controlled accurately and operated easily with a steady performance.No germs were grown in the culture medium after 5 days＇ running.The preliminary results showed that after the tissue engineering bones were cultured in the bioreactor for 10 days,cell proliferation and ALP activity in this perfusion culture and loading group were significantly higher than those in the static culture group and the simple perfusion culture group.Conclusions The bioreactor could be an ideal dynamic culture and loading device for biomechanical study of tissue engineering bone.

关 键 词：生物反应器 骨组织工程 生物力学 细胞培养 压应变 动态载荷 

分 类 号：R318.01[医药卫生—生物医学工程]