血凝素样氧化型低密度脂蛋白受体1和白细胞介素1β在人粥样硬化冠状动脉中的表达及与动脉粥样硬化进展的关系  

Expression of Lectin-Like Oxidized Low Density Lipoprotein Receptor-1 and Interleukin-1β in Human Coronary Artery and Their Relationship with Atherosclerosis Development

作　　者：魏静静[1,2] 孙雷[1] 张树辉[2] 李静[1] 于霄[1] 

机构地区：[1]大连医科大学病理学教研室,辽宁省大连市1160442 [2]上海中医药大学附属岳阳医院病理科,上海市200437

出　　处：《中国动脉硬化杂志》2011年第11期901-905,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的观察人动脉粥样硬化冠状动脉血凝素样氧化型低密度脂蛋白受体1(LOX-1)和白细胞介素1β(IL-1β)表达及相互关系。方法以63例尸检标本中获得的冠状动脉标本为研究对象,包括15例正常冠状动脉、26例冠状动脉狭窄程度≤50%和22例冠状动脉狭窄程度>50%,用免疫组织化学的方法检测LOX-1、IL-β和CD68和平滑肌肌动蛋白在各组冠状动脉中的表达情况。结果正常冠状动脉LOX-1、IL-β呈阴性表达,粥样硬化冠状动脉LOX-1、IL-1β的表达阳性率分别为47.5%(23/48)和18.8%(9/48),两者主要表达于内皮细胞、巨噬细胞、平滑肌细胞和泡沫细胞。冠状动脉狭窄程度≤50%组斑块(61.5%)LOX-1的表达要高于正常组(0)和狭窄程度>50%组(31.8%,P=0.029),LOX-1在动脉粥样硬化冠状动脉内膜的表达强度要高于中膜(P=0.044),且与IL-β表达呈正相关(r=0.519,P=0.001)。结论 LOX-1主要表达于动脉粥样硬化病变进展初期的内皮细胞、巨噬细胞、平滑肌细胞和泡沫细胞,LOX-1在内膜中的作用要强于中膜。LOX-1与IL-1β表达成正相关提示其共同作用参与动脉粥样硬化的发生发展。Aim To study the expression of lectin-like oxidized low-density lipoprotein receptor-1（LOX-1） and interleukin-1β（IL-1β） in normal human coronary artery and atherosclerosis and the relationship between LOX-1 and IL-1β.Methods Immunohistochemistry was used to detect the expression of LOX-1,IL-1β,CD68 and smooth muscle actin on the tissue containing 15 normal coronary artery,48 atherosclerotic coronary arteries.Results The expression of LOX-1 and IL-1β in normal coronary artery was negative.Positive expression rates of LOX-1 and IL-1β were 47.5%（23/48） and 18.8%（9/48） in atherosclerotic coronary arteries,respectively.The expression mainly locates in the membrane of endothelial cells,macrophages smooth muscle cells and foam cells.Positive expression rates of LOX-1 were higher in atherosclerosis coronary artery with stenosis≤50% group（61.5%） than stenosis50% group（31.8%）.The expression of LOX-1 in intima was stronger than in media（P=0.044）,and a positive association existed between LOX-1 and IL-1β in atherosclerosis（r=0.519,P=0.001）.Conclusions Besides endothelial cells,LOX-1 is also expressed on smooth muscle cells and monocyte/macrophages,although the expression level is relatively low.It may appear in the early stages of atherogenesis.Positive association between LOX-1 and IL-1β in atherosclerotic coronary artery suggests that they play an important role in development and progression of atherosclerosis.

关 键 词：血凝素样氧化型低密度脂蛋白受体 白细胞介素1Β 冠状动脉粥样硬化 动脉粥样斑块 

分 类 号：R363[医药卫生—病理学]