血小板源生长因子对大鼠血管平滑肌细胞表达基质金属蛋白酶2的影响  

Effects of Platelet-derived Growth Factor on Expression of Matrix Metalloproteinases-2 in Vascular Smooth Muscle Cell of Rat

作　　者：于悦卿[1] 郭文潮[2] 郝玉宾[3] 

机构地区：[1]河北省人民医院检验科,河北省石家庄市050051 [2]河北省人民医院三优中心,河北省石家庄市050051 [3]河北省人民医院临床医学研究中心,河北省石家庄市050051

出　　处：《中国动脉硬化杂志》2011年第11期911-914,918,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的研究血小板源生长因子对血管平滑肌细胞迁移的影响和细胞产生基质金属蛋白酶2的变化。方法体外培养的血管平滑肌细胞经血小板源生长因子处理0 min、20 min和6 h后进行如下检测:迁移实验观察细胞在盖玻片上的迁移情况;明胶酶谱检测血管平滑肌细胞分泌基质金属蛋白酶2活性;基因芯片和逆转录-聚合酶链反应技术检测血管平滑肌细胞表达基质金属蛋白酶2 mRNA的变化。结果细胞迁移实验显示随着作用时间的延长,血小板源生长因子明显促进细胞的迁移,血小板源生长因子处理20 min和6 h后迁移距离(分别为4.9±0.5、7.1±1.2μm)是对照组(2.3±0.15μm)的2.13倍和3.09倍,差异有显著性(P<0.05);明胶酶谱测定表明:血小板源生长因子显著提高血管平滑肌细胞分泌的基质金属蛋白酶2的活性,作用20 min与6 h后(分别为480.7±27.3和851.5±56.4)是对照组(296.7±15.8)的1.62倍和2.87倍(P<0.01);血小板源生长因子对血管平滑肌细胞表达基质金属蛋白酶2有明显的诱导作用,作用20 min与6 h后的表达(分别为0.54±0.09和0.77±0.04)是对照组(0.35±0.04)的1.54倍和2.2倍(P<0.05)。结论血小板源生长因子明显诱导血管平滑肌细胞表达基质金属蛋白酶2,促进血管平滑肌细胞迁移。Aim To investigate the effects of platelet-derived growth factor（PDGF） on migration and matrix metalloproteinases-2（MMP-2） expression in vascular smooth muscle cells（VSMC）.Methods After treated with PDGF 0 min,20 min and 6 hours,the ability of cell migration was measured using microscope,and MMP-2 activity was assayed by gel zymography.Furthermore the expression of MMP-2 was screened by using Chip and confirmed by reverse transcription polymerase chain reaction（RT-PCR）.Results The ability of cell migration was improved by PDGF.It was 2.13-fold for 20 min treatment and 3.09-fold for 6 h treatment compared to without PDGF treatment,respectively（P0.05,n=3）.The activity of MMP-2 was also significantly increased 1.62-fold and 2.87-fold for 20 min and 6 h treated by PDGF compared with 0 min,respectively（P0.01,n=3）.The mRNA expression of MMP-2 was 1.54-fold,2.2-fold higher in cultured with PDGF at 20 min and 6 h than 0 min,respectively（P0.05,n=3）.Conclusion Our data indicate that PDGF improve vascular smooth muscle cell migration via inducing MMP-2 expression and enhancing MMP-2 activity.

关 键 词：炎症反应 血小板源生长因子 血管平滑肌细胞 基质金属蛋白酶2 

分 类 号：R363[医药卫生—病理学]