人乳腺癌MCF-7他莫昔芬耐药细胞株的建立及鉴定  

Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer

作　　者：莫志强[1] 涂刚[1] 罗浩军[1] 李振华[1] 杨光伦[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌乳腺外科,重庆400016

出　　处：《第三军医大学学报》2011年第21期2256-2259,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81072149)~~

摘　　要：目的构建人乳腺癌细胞MCF-7他莫昔芬(Tamoxifen,TAM)耐药细胞模型,并鉴定其耐药性。方法采用高浓度短时间4-羟他莫昔芬(4-hydroxytamoxifen,OHT)冲击法,诱导人乳腺癌MCF-7/TAM耐药细胞株。通过细胞形态学观察、生长曲线、细胞倍增时间、药物毒性实验、细胞周期及凋亡相关蛋白BIK和Caspase-7的检测,评价MCF-7/TAM耐药细胞株的耐药性。结果成功构建MCF-7/TAM耐药细胞株,其耐药指数(resistance index,RI)为(13.88±2.93)(P<0.05)。与其母细胞相比,MCF-7/TAM耐药细胞株不仅细胞形态发生了变化,生长增殖能力增强(P<0.05),细胞倍增时间缩短(P<0.05),而且对OHT介导的毒性效应有较强的抵抗能力(P<0.05),OHT不能促进MCF-7/TAM耐药细胞内BIK和Caspase-7蛋白的表达(P<0.05)。结论通过高浓度短时间OHT冲击法诱导的MCF-7/TAM耐药细胞株对TAM产生一定的耐药性。Objective To establish a Tamoxifen（TAM）-resistant cell line of MCF-7 human breast cancer and to investigate the drug resistance of the cell line.Methods MCF-7 Tamoxifen-resistant（MCF-7/TAM-R） cell line was induced by a high concentration of 4-hydroxytamoxifen（OHT） treatment for a short period.Drug resistance of MCF-7/TAM-R cells were determined by cell morphology,cell growth curve,cell doubling time,drug toxicity tests,cell cycle and expression of apoptosis-related proteins BIK and Caspase-7.Results MCF-7/TAM-R cell line was successfully established,and its drug resistance index（RI） was 13.88±2.93（P〈0.05）.Compared with its parental cell line,MCF-7/TAM-R cell line exhibited a different cell morphology,a higher proliferation ability（P〈0.05）,a shorter cell doubling time（P〈0.05） and a stronger resistance to OHT-mediated cytotoxic effects（P〈0.05）.OHT failed to induce the expression of BIK and Caspase-7（P〈0.05） in MCF-7/TAM-R cell line.Conclusion MCF-7/TAM-R cell line induced by a high concentration of OHT for a short period has a significant resistance to TAM.

关 键 词：乳腺癌 他莫昔芬 耐药细胞株 

分 类 号：R73-354[医药卫生—肿瘤] R734.2[医药卫生—临床医学]